Adhesion of bovine endothelial cells on fibronectin and collagen before and after
nonenzymatic glycation in vitro has been studied. Nonenzymatic glycation of these
proteins reduced their ability to bind endothelial cells. Furthermore, nonenzymatically
glycated fibronectin failed to bind to normal and nonenzymatically glycated gelatin
and to fibrin. So gelatin and fibrin Sepharoses can be used to separate highly glycated
fibronectins from fibronectins with a low degree of nonenzymatic glucose substitution.
Sodium dodecylsulfate polyacrylamide gel electrophoresis did not demonstrate a covalent
cross-link between nonenzymatically glycated fibronectins.
These results present further evidences for the role of nonenzymatic glycation of
proteins in the development of vascular complications in long-term diabetes and of
atherosclerosis.
Nonenzymatic glycation - Endothelial cells - Fibronectin - Collagen - Gelatin
