The Influence of Acute Resistance Training and Body Composition on Coagulation and Fibrinolytic Activity in Low-Risk Women

Abstract

This study explored the coagulation and fibrinolytic responses to acute resistance training in young women and aimed to determine the influence of body composition on these variables. Healthy young women aged 23±5 yrs participated in the study. Body fat and fat distribution were assessed using DEXA. Each subject performed 6 sets of 10 leg extension repetitions at an intensity associated with 70% of 1-repetition maximum. Markers of coagulation (TAT), fibrinolytic stimulation (tPA) and inhibition (PAI-1) were assessed before and immediately after exercise. tPA activity increased in response to acute resistance exercise (p<0.05), however, there was no change in TAT or PAI-1 activity. Percent body fat was negatively correlated to the tPA response to exercise (r=−0.44), and positively related to PAI-1 at baseline (r=0.47) and post-exercise (r=0.47), and to post-exercise TAT (r=0.44). Android/gynoid fat ratio was negatively related to post-exercise tPA (r=−0.43), positively related to PAI-1 at baseline (r=0.61) and post-exercise (r=0.62) and to post-exercise TAT (r=0.43). These physiological responses suggest women with elevated body fat may be at increased risk of an adverse thrombosis-related event both at rest and during exercise compared to leaner women.

tissue plasminogen activator - plasminogen activator inhibitor - thrombin - antithrombin

References

• 1 ACSM .Muscular fitness and assessment. In: ACSM's Resource Manual for Exercise Testing and Prescription. Baltimore, MD: Lippincott, Williams & Wilkins; 2009: 332-348
• 2 Ahmadizad S, El-Sayed MS. The acute effects of resistance exercise on the main determinants of blood rheology.  J Sports Sci. 2005;  23 243-249
• 3 Ahmadizad S, El-Sayed MS. The effects of graded resistance exercise on platelet aggregation and activation.  Med Sci Sports Exerc. 2003;  35 1026-1032
• 4 Andrew M, Carter C, O’Brodovich H, Heigenhauser G. Increases in factor VIII complex and fibrinolytic activity are dependent on exercise intensity.  J Appl Physiol. 1986;  60 1917-1922
• 5 Ardissino D, Merlini PA, Eisenberg PR, Kottke-Marchant K, Crenshaw BS, Granger CB. Coagulation markers and outcomes in acute coronary syndromes.  Am Heart J. 1998;  136 S7-S18
• 6 Beisiegel B, Treese N, Hafner G, Meyer J, Darius H. Increase in endogenous fibrinolysis and platelet activity during exercise in young volunteers.  Agents Actions Suppl. 1992;  37 183-189
• 7 Billaut F, Smith K. Sex alters impact of repeated bouts of sprint exercise on neuromuscular activity in trained athletes.  Appl Physiol Nutr Metab. 2009;  34 689-699
• 8 Botcazou M, Jacob C, Gratas-Delamarche A, Vincent S, Bentue-Ferrer D, Delamarche P, Zouhal H. Sex effect on catecholamine responses to sprint exercise in adolescents and adults.  Pediatr Exerc Sci. 2007;  19 132-144
• 9 Cigolini M, Targher G, Bergamo Andreis IA, Tonoli M, Agostino G, De Sandre G. Visceral fat accumulation and its relation to plasma hemostatic factors in healthy men.  Arterioscler Thromb Vasc Biol. 1996;  16 368-374
• 10 Clark BC, Collier SR, Manini TM, Ploutz-Snyder LL. Sex differences in muscle fatigability and activation patterns of the human quadriceps femoris.  Eur J Appl Physiol. 2005;  94 196-206
• 11 Cooper JA, Nagelkirk PR, Coughlin AM, Pivarnik JM, Womack CJ. Temporal changes in tPA and PAI-1 after maximal exercise.  Med Sci Sports Exerc. 2004;  36 1884-1887
• 12 deJong AT, Womack CJ, Perrine JA, Franklin BA. Hemostatic responses to resistance training in patients with coronary artery disease.  J Cardiopulm Rehabil. 2006;  26 80-83
• 13 El-Sayed MS. Fibrinolytic and hemostatic parameter response after resistance exercise.  Med Sci Sports Exerc. 1993;  25 597-602
• 14 Focht BC, Koltyn KF. Influence of resistance exercise of different intensities on state anxiety and blood pressure.  Med Sci Sports Exerc. 1999;  31 456-463
• 15 Gebara OC, Mittleman MA, Sutherland P, Lipinska I, Matheney T, Xu P, Welty FK, Wilson PW, Levy D, Muller JE. Association between increased estrogen status and increased fibrinolytic potential in the Framingham Offspring Study.  Circulation. 1995;  91 1952-1958
• 16 Giardina EG, Chen HJ, Sciacca RR, Rabbani LE. Dynamic variability of hemostatic and fibrinolytic factors in young women.  J Clin Endocrinol Metab. 2004;  89 6179-6184
• 17 Gonzales JU, Parker BA, Ridout SJ, Smithmyer SL, Proctor DN. Femoral shear rate response to knee extensor exercise: an age and sex comparison.  Biorheology. 2009;  46 145-154
• 18 Harriss DJ, Atkinson G. International Journal of Sports Medicine – Ethical Standards in Sport and Exercise Science Research.  Int J Sports Med. 2009;  30 701-702
• 19 Helenius G, Hagvall SH, Esguerra M, Fink H, Soderberg R, Risberg B. Effect of shear stress on the expression of coagulation and fibrinolytic factors in both smooth muscle and endothelial cells in a co-culture model.  Eur Surg Res. 2008;  40 325-332
• 20 Hittel DS, Kraus WE, Hoffman EP. Skeletal muscle dictates the fibrinolytic state after exercise training in overweight men with characteristics of metabolic syndrome.  J Physiol. 2003;  548 401-410
• 21 Janand-Delenne B, Chagnaud C, Raccah D, Alessi MC, Juhan-Vague I, Vague P. Visceral fat as a main determinant of plasminogen activator inhibitor 1 level in women.  Int J Obes Relat Metab Disord. 1998;  22 312-317
• 22 Jiang X, Wang Y, Hand AR, Gillies C, Cone RE, Kirk J, O’Rourke J. Storage and release of tissue plasminogen activator by sympathetic axons in resistance vessel walls.  Microvasc Res. 2002;  64 438-447
• 23 Junker R, Heinrich J, Schulte H, van de Loo J, Assmann G. Coagulation factor VII and the risk of coronary heart disease in healthy men.  Arterioscler Thromb Vasc Biol. 1997;  17 1539-1544
• 24 Kalaria VG, Zareba W, Moss AJ, Pancio G, Marder VJ, Morrissey JH, Weiss HJ, Sparks CE, Greenberg H, Dwyer E, Goldstein R, Watelet LF. Gender-related differences in thrombogenic factors predicting recurrent cardiac events in patients after acute myocardial infarction. The THROMBO Investigators.  Am J Cardiol. 2000;  85 1401-1408
• 25 Landin K, Stigendal L, Eriksson E, Krotkiewski M, Risberg B, Tengborn L, Smith U. Abdominal obesity is associated with an impaired fibrinolytic activity and elevated plasminogen activator inhibitor-1.  Metabolism. 1990;  39 1044-1048
• 26 Larsson PT, Wallen NH, Egberg N, Hjemdahl P. Alpha-adrenoceptor blockade by phentolamine inhibits adrenaline-induced platelet activation in vivo without affecting resting measurements.  Clin Sci (Lond). 1992;  82 369-376
• 27 Lee KW, Lip GY. Effects of lifestyle on hemostasis, fibrinolysis, and platelet reactivity: a systematic review.  Arch Intern Med. 2003;  163 2368-2392
• 28 Lin X, El-Sayed MS, Waterhouse J, Reilly T. Activation and disturbance of blood haemostasis following strenuous physical exercise.  Int J Sports Med. 1999;  20 149-153
• 29 Morris PJ, Packianathan CI, Van Blerk CJ, Finer N. Moderate exercise and fibrinolytic potential in obese sedentary men with metabolic syndrome.  Obes Res. 2003;  11 1333-1338
• 30 Mustonen P, Lepantalo M, Lassila R. Physical exertion induces thrombin formation and fibrin degradation in patients with peripheral atherosclerosis.  Arterioscler Thromb Vasc Biol. 1998;  18 244-249
• 31 Prisco D, Paniccia R, Guarnaccia V, Olivo G, Taddei T, Boddi M, Gensini GF. Thrombin generation after physical exercise.  Thromb Res. 1993;  69 159-164
• 32 Prowse CV, Cash JD. Physiologic and pharmacologic enhancement of fibrinolysis.  Semin Thromb Hemost. 1984;  10 51-60
• 33 Szymanski LM, Pate RR. Fibrinolytic responses to moderate intensity exercise. Comparison of physically active and inactive men.  Arterioscler Thromb. 1994;  14 1746-1750
• 34 Tofler G, Stone P, Maclure M, Edelman E, Davis V, Robertson T, Antman E, Muller J. Analysis of possible triggers of acute myocardial infarction.  Am J Cardiol. 1990;  66 22-27
• 35 Van Beaumont W. Evaluation of hemoconcentration from hematocrit measurements.  J Appl Physiol. 1972;  32 712-713
• 36 Womack CJ, Nagelkirk PR, Coughlin AM. Exercise-induced changes in coagulation and fibrinolysis in healthy populations and patients with cardiovascular disease.  Sports Med. 2003;  33 795-807

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