Abstract
Extracts of the traditional medicinal herb Andrographis paniculata and its main constituents are described in the literature as showing anticancer activity.
The aim of this study was to isolate the main constituents of a commercially available
phytotherapeutic preparation of A. paniculata and to determine their chemosensitizing potential in a leukemia cell line. Chromatographic
separation steps resulted in the isolation of the diterpenes andrographolide (1) and 14-deoxy-11,12-didehydroandrographolide (3) and the diterpene glucoside neoandrographolide (2). The combination of these constituents with suboptimal concentrations of etoposide
revealed compound 2 as chemosensitizer in S-Jurkat and X chromosome-linked inhibitor of apoptosis protein
(XIAP)-overexpressing Jurkat cells, a model for chemoresistance.
Key words
Andrographis paniculata
- Acanthaceae - neoandrographolide - chemosensitizer - etoposide
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Prof. Dr. Hermann Stuppner
Institute of Pharmacy/Pharmacognosy
Center for Molecular Biosciences Innsbruck
Leopold-Franzens University of Innsbruck
Innrain 52c, Josef-Moeller-Haus
6020 Innsbruck
Austria
Phone: + 43 51 25 07 53 00
Fax: + 43 51 25 07 29 39
Email: hermann.stuppner@uibk.ac.at
