Planta Med 2011; 77(4): 334-339
DOI: 10.1055/s-0030-1250386
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

The Anti-Immobility Effect of Hyperoside on the Forced Swimming Test in Rats is Mediated by the D2-Like Receptors Activation

Juliana Schulte Haas1 , Eveline Dischkaln Stolz2 , Andresa Heemann Betti2 , Ana Cristina Stein2 , Jan Schripsema3 , Gilsane Lino von Poser1 , Stela Maris Kuze Rates2
  • 1Laboratório de Farmacognosia, Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
  • 2Laboratório de Psicofarmacologia Experimental, Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
  • 3Grupo Metabolômica, LCQUI/CCT, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Brazil
Further Information

Publication History

received February 24, 2010 revised August 27, 2010

accepted Sept. 6, 2010

Publication Date:
13 October 2010 (online)


The crude extracts of Hypericum species native to South Brazil showed analgesic and antidepressant-like effects in rodents. The chemical characterization of these species revealed that they are rich in flavonoids and phloroglucinol derivatives. In the present study a detailed investigation was performed on the activities of hyperoside (HYP), a common flavonoid in the genus Hypericum. Hyperoside was obtained from the aerial parts of H. caprifoliatum by chromatographic procedures. Mice treated with single doses (10, 20 and 40 mg/kg i.p.) did not present signs of toxicity or weight loss. At 20 and 40 mg/kg i.p. the mice exploratory behavior in the open field test was reduced. At 20 mg/kg i. p. the pentobarbital sleeping time increased, but not the sleeping latency. No activity was found on the hot-plate (10 and 20 mg/kg i.p.) or in the acetic acid-induced writhing test (20 and 40 mg/kg p.o.). Nevertheless, an antidepressant-like effect in the forced swimming test in mice and rats was observed (HYP 10 and 20 mg/kg i.p. in mice; HYP 1.8 mg/kg/day p.o. in rats). The antidepressant-like effect in rats was prevented by the administration of sulpiride (50 mg/kg i.p.) a D2 antagonist. In conclusion, hyperoside was found to present a depressor effect on the central nervous system as well as an antidepressant-like effect in rodents which is, at least in part, mediated by the dopaminergic system.


Prof. Dr. Stela Maris Kuze Rates

Programa de Pós-graduação em Ciências Farmacêuticas
Universidade Federal do Rio Grande do Sul

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90610-000 Porto Alegre RS


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