Horm Metab Res 2011; 43(1): 43-47
DOI: 10.1055/s-0030-1265220
Animals

© Georg Thieme Verlag KG Stuttgart · New York

Glycerol-3-phosphate Dehydrogenase Expression and Oxygen Consumption in Liver Mitochondria of Female and Male Rats with Chronic Alteration of Thyroid Status

H. Rauchová1 , 2 , T. Mráček1 , 3 , P. Novák1 , M. Vokurková1 , 2 , T. Soukup1
  • 1Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
  • 2Center for Cardiovascular Research, Prague, Czech Republic
  • 3Center for Applied Genomics, Prague, Czech Republic
Further Information

Publication History

received 01.07.2010

accepted 23.08.2010

Publication Date:
30 September 2010 (eFirst)

Abstract

In our chronic experiments (over several months), the activity and protein amount of glycerol-3-phosphate dehydrogenase (GPDH) in mitochondria isolated from the liver of adult male and female inbred Lewis strain euthyroid (EU), hyperthyroid (TH), and hypothyroid (HY) rats were analyzed by biochemical and Western blot methods. The TH status was induced by intraperitoneal injections of 3,3′,5-triiodo-L-thyronine and the HY status with 0.05% solution of methimazole in drinking water. The TH status led to a significant increase and the HY status to a significant decrease of enzyme activity and protein amount in both male and female animals. These changes were, however, more pronounced in females. The EU and TH female rats also showed a significantly higher activity and the TH female rats showed also a significantly higher enzyme amount in comparison with males, while the HY rats showed low levels in both sexes. The glycerol-3-phosphate-dependent oxygen consumption of freshly isolated rat liver mitochondria from the TH animals was higher in comparison with the EU animals and it was activated by idebenone, a synthetic analogue of coenzyme Q, in both the EU and TH rats. Measurements of serum thyroid hormone levels and analysis of anatomical parameters (relative heart and thyroid gland weights) confirmed that our procedures inducing the TH and HY states are efficient and reliable and that determination of GPDH can serve as an additional criterion for the evaluation of the thyroid hormone status.

References

Correspondence

H. Rauchová

Institute of Physiology, v.v.i.

Academy of Sciences of the

Czech Republic

Vídeňská 1083

42220 Prague 4

Czech Republic

Phone: +420/2/4106 2432

Fax: +420/2/4106 2488

Email: rauchova@biomed.cas.cz