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Planta Med 2011; 77(16): 1788-1793
DOI: 10.1055/s-0030-1271157
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Cytotoxicity of Ethanolic Extracts of Artemisia annua to Molt-4 Human Leukemia Cells

Narendra P. Singh1 , Jorge F. S. Ferreira2 , Ji Sun Park1 , Henry C. Lai1
  • 1Department of Bioengineering, University of Washington, Seattle, WA, USA
  • 2Appalachian Farming Systems Research Center, USDA-ARS, Beaver, WV, USA
Further Information

Publication History

received February 2, 2011 revised April 29, 2011

accepted May 5, 2011

Publication Date:
14 June 2011 (online)

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Abstract

Although dihydroartemisinin (DHA) and other artemisinin derivatives have selective toxicity towards cancer cells, Artemisia annua (A. annua) extracts containing artemisinin have not been evaluated for their anticancer potential. Our main goal was to assess the anticancer effect of ethanolic leaf extracts of A. annua from Brazilian and Chinese origins (with DHA as a comparison) on normal and cancer cells. Leukocytes and leukemia (Molt-4) cells were counted at 0, 24, 48, and 72 hr after treatment with extracts having artemisinin concentrations of 0, 3.48, 6.96, and 13.92 µg/mL. Also, we assessed the antioxidant capacity of these extracts using the oxygen radical absorbance capacity (ORAC) test. Both extracts had high antioxidant capacity and toxicity towards Molt-4 cells. DHA was significantly more potent (p < 0.05) in killing Molt-4 cells than Brazilian extract at 48 and 72 hr and Chinese extract at 72 hr. In Molt-4 cells, LD50 values for Brazilian and Chinese extracts were comparable at all time points and not significantly different from DHA at 24 hr. In leukocytes, DHA, Chinese extract, and Brazilian extract had LD50 values of 760.42, 13.79, and 28.23 µg/mL of artemisinin, respectively, indicating a better safety index for the Brazilian extract compared to that of the Chinese extract at 24 hr. However, at 48 and 72 hr, the toxicity in leukocytes for any of the treatment groups was not significantly different. These experiments suggest that these extracts may have potential application in cancer treatment.

Key words

Artemisia annua - Asteraceae - ethanolic extracts - antioxidant - flavonoids - synergism - leukemia - MOLT-4

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Dr. Narendra P. Singh

Department of Bioengineering
University of Washington

Box 355061

Seattle, WA 98195-5061

USA

Phone: +1 206 68 5 20 60

Fax: +1 206 68 5 39 25

Email: narendra@u.washington.edu

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