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Z Gastroenterol 2011; 49(6): 737-739
DOI: 10.1055/s-0031-1273392
Übersicht

© Georg Thieme Verlag KG Stuttgart · New York

Use of Isolated Liver Perfusion in Metabolic Studies: Ground-laying Work in Experimental Hepatology

Leberperfusion für Stoffwechselstudien: grundlegende Arbeiten in Experimenteller HepatologieH. Sies1 , 2
  • 1Institute of Biochemistry and Molecular Biology I, Heinrich-Heine-University Düsseldorf, Germany
  • 2College of Science, King Saud University, Riyadh, Saudi Arabia
Further Information

Publication History

manuscript received: 23.2.2011

manuscript accepted: 26.4.2011

Publication Date:
01 June 2011 (online)

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Zusammenfassung

Die moderne Stoffwechselforschung in der Hepatologie verwendet nicht invasive Techniken am ganzen Organismus sowie am intakten Organ. Nach Dekaden der Zellkultur und des Studiums der Eigenschaften isolierter Zellen rückt nun die Untersuchung der räumlich-zeitlichen Organisation der verschiedenen Zelltypen in den Vordergrund unter Verwendung von Modellen intakter Organe und Gewebe. Dies betrifft besonders die Leber als das wesentliche Stoffwechselorgan. Dieser kurze Artikel illustriert Vorteile und Grenzen solcher Ansätze und fokussiert auf ausgewählte Aspekte aus dem Bereich des Stoffwechsels von Ammonium/Harnstoff und Glutamin.

Abstract

Modern metabolic research in hepatology employs non-invasive techniques of the whole organism, and it includes studying the intact organ. Following recent decades of efforts in culturing isolated cells and studying their properties separately, it has become clear that the spatiotemporal organisation of different cell types in a tissue requires studies using models of the intact organ or tissue. This applies particularly to the liver as the major organ of metabolic transformation and activity. The present brief article attempts to illustrate the advantages and limitations of such approaches, focusing on selected aspects of ammonia/urea and glutamine metabolism as an example.

Schlüsselwörter

Leber - Ammoniak - Perfusion

Key words

liver - ammonia - perfusion

References

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Prof. Dr. Helmut Sies

Institute of Biochemistry and Molecular Biology I, Heinrich-Heine-Universität Düsseldorf

Universitätsstraße 1, Bldg 22.03

40225 Düsseldorf

Germany

Phone: ++ 49/2 11/8 11 59 56

Fax: ++ 49/2 11/8 11 59 80

Email: sies@uni-duesseldorf.de

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