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Horm Metab Res 2011; 43(8): 537-544
DOI: 10.1055/s-0031-1277227
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Effects of Adiponectin in TNF-α, IL-6, and IL-10 Cytokine Production from Coronary Artery Disease Macrophages

E. Kyriazi1 , 2 , P. C. Tsiotra1 , E. Boutati2 , I. Ikonomidis3 , K. Fountoulaki3 , E. Maratou1 , J. Lekakis3 , G. Dimitriadis2 , D. T. Kremastinos3 , S. A. Raptis1 , 2
  • 1Hellenic National Center for the Research, Prevention and Treatment of Diabetes Mellitus and its Complications (H.N.D.C), Athens, Greece
  • 22nd Department of Internal Medicine, Research Institute and Diabetes Center, Athens University Medical School, “Attikon” University General Hospital, Athens, Greece
  • 32nd Department of Cardiology, Athens University Medical School, “Attikon” University General Hospital, Athens, Greece
Further Information

Publication History

received 04.11.2011

accepted 19.04.2011

Publication Date:
30 May 2011 (online)

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Abstract

Adiponectin, an adipose tissue secreted protein, exhibits anti-inflammatory and antiatherogenic properties. We examined the effects of the globular and full-length adiponectin on cytokine production in macrophages derived from Coronary Artery Disease (CAD) patients and control individuals. Adiponectin's effects in human macrophages upon lipopolysaccharide (LPS) treatment were also examined. Full length adiponectin acted differently on TNF-α and IL-6 production by upregulating TNF-α and IL-6 protein production, but not their mRNA expression. Additionally, full length adiponectin was unable to abrogate LPS proinflammatory effect in TNF-α and IL-6 mRNA expression in CAD and NON-CAD macrophages. In contrast, globular adiponectin appeared to have proinflammatory properties by potently upregulating TNF-α and IL-6 mRNA and protein secretion in human macrophages while subsequently rendered cells resistant to further proinflammatory stimuli. Moreover, both forms of adiponectin powerfully suppressed scavenger MSR-AI mRNA expression and augmented IL-10 protein release, both occurring independently of the presence of LPS or CAD. These data indicate that adiponectin could potentially protect human macrophages via the elevated IL-10 secretion and the suppression of MSR-AI expression. It can also be protective in CAD patients since the reduced adiponectin-induced IL-6 release in CAD macrophages compared to controls, could be beneficial in the development of inflammation related atherosclerosis.

Key words

proinflammatory cytokines - atherosclerosis - adiponectin - macrophages

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Correspondence

P. C. TsiotraPhD 

Molecular Biology Lab.

Hellenic National Center for the

Research, Prevention and

Treatment of Diabetes Mellitus

and its Complications (H.N.D.C)

Ploutarchou 3

10675 Athens

Greece

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