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Geburtshilfe Frauenheilkd 2012; 72(1): 70-74
DOI: 10.1055/s-0031-1280433
Original Article
GebFra Science
Georg Thieme Verlag KG Stuttgart · New York

The Effect of High Dose Methylprednisolone on Experimental Ovarian Torsion/Reperfusion Injury in Rats

Die Wirkung von hochdosiertem Methylprednisolon auf experimentelle Ovarialtorsion/Reperfusion in Ratten
M. A. Osmanağaoğlu
1   Department of Obstetrics and Gynecology, Karadeniz Technical University, Trabzon, Turkey
,
M. Kesim
2   Department of Pharmacology, Karadeniz Technical University, Trabzon, Turkey
,
E. Yuluğ
3   Department of Histology and Embryology, Karadeniz Technical University, Trabzon, Turkey
,
A. Menteşe
4   Department of Biochemistry, Karadeniz Technical University, Trabzon, Turkey
,
C. S. Karahan
4   Department of Biochemistry, Karadeniz Technical University, Trabzon, Turkey
› Author Affiliations
Further Information

Publication History

received 17 September 2011
revised 13 November 2011

accepted 1 03 November 2011

Publication Date:
07 February 2012 (online)

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Abstract

Purpose: Aim of the study was to evaluate the effects of high dose methylprednisolone on experimental ovarian torsion-detorsion injury in rats. Materials and Methods: Twenty-two Sprague-Dawley rats were randomly divided into three groups. Group 1 (ischemia group, 8 rats) were subjected to left adnexal torsion for 2 h but received no treatment. Group 2 (methylprednisolone group, 8 rats) were subjected to left adnexal torsion for 2 h and received methylprednisolone (30 mg/kg, administered intraperitoneally) at the end of a 2-hour ischemic period followed by 24-hour reperfusion. Group 3 (control group, 6 rats) underwent a sham operation with no adnexal torsion and no treatment. Results: Serum malondialdehyde (MDA), ischemia-modified albumin (IMA), total oxidant status (TOS) and tissue MDA levels were increased in Group 1 rats; total antioxidant status (TAS) levels and oxidative stress index (OSI) were significantly decreased compared with rats in Groups 2 and 3 (p < 0.05). MDA, IMA, TOS and tissue MDA levels were lower and TAS levels and OSI were higher in Group 3 compared to Group 2. Ovarian damage scores in Group 1 were significantly higher compared with Groups 2 and 3 (p < 0.05). Conclusion: This study demonstrated that high dose methylprednisolone reduces ovarian ischemia/reperfusion injury.

Zusammenfassung

Fragestellung: Ziel der Studie war es, die Effekte von hohen Dosen Methylprednisolon auf experimentelle ovariale Torsion-Detorsions-Verletzungen bei Ratten zu bewerten. Material und Methoden: 22 Sprague-Dawley-Ratten wurden in 3 Gruppen randomisiert. In Gruppe 1 (Ischämiegruppe, 8 Ratten) wurde der linke Adnex 2 h einer Torsion unterzogen, es erfolgte keine Behandlung. In Gruppe 2 (Methylprednisolon-Gruppe, 8 Ratten) wurde nach 2-stündiger Torsion des linken Adnexes und der damit verbundenen Ischämie Methylprednisolon (30 mg/kg, intraperitoneal) verabreicht, es folgte eine 24-Stunden-Reperfusion. Gruppe 3 (Sham-Operationsgruppe als Kontrollgruppe, 6 Ratten) blieb ohne Torsion des Adnexes oder Behandlung. Ergebnisse: Serum-Malondialdehyd (MDA), Ischämie-modiziertes Albumin (IMA), Gesamtoxydationsmittelstatus (TOS) und MDA-Level in Gewebe waren in den Gruppe-1-Ratten erhöht; das Niveau des Gesamtantioxydationsmittelstatus (TAS) und der Index des oxidativen Stresses (OSI) waren im Vergleich zu Gruppe-2- und Gruppe-3-Ratten signifikant verringert (p < 0,05). In Gruppe 3 waren die MDA-, IMA-, TOS- und Gewebe-MDA-Level niedriger und die TAS- und OSI-Level höher als in Gruppe 2. Der Grad des resultierenden ovarialen Schadens war in Gruppe 1 signifikant höher als in Gruppe 2 und 3 (p < 0,05). Schlussfolgerung: Diese Studie demonstrierte, dass hoch dosiertes Methylprednisolon ovariale Ischämie-/Reperfusions-Verletzungen reduziert.

Key words

high dose methylprednisolone - rats - ovarian ischemia-reperfusion - oxidative stres - histopathology

Schlüsselwörter

hoch Dosis Methylprednisolon - Ratten - Ovarial Ischämie-Reperfusion - oxidativ Stress - Histopathologie
 

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