Drimane-type Sesquiterpenes with a Dioxabicyclooctane Skeleton from the Fruiting Bodies of Nigrofomes melanoporus and Their Cytotoxicity

Abstract

Two new drimane-type compounds, nigrofomins A (1) and B (2), possessing a rare dioxabicyclooctane moiety, were purified from the fruiting bodies of Nigrofomes melanoporus. Their structures were determined using 1D-, 2D-NMR and HR-ESI-MS spectroscopic analyses. In addition, 1 was established by X-ray crystallographic studies. Both nigrofomins A (1) and B (2) exhibited cytotoxicity on acute T-cell leukemia (Jurkat), human nasopharyngeal carcinoma (NPC-TW01), and lung cancer (NCI-H661) cells with IC₅₀ values in the range of 99.44–246.32 µM. Furthermore, the effects of 1 and 2 on cell-cycle progression of Jurkat cells displayed a concentration-dependent accumulation in the G₀/G₁ phase.

Key words

Nigrofomes melanoporus - Polyporaceae - drimane - sesquiterpenes - cytotoxicity

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1 These authors provided equal contributions to this work.