A sensitive and selective high performance liquid chromatographic (HPLC) method was
developed and validated for quantification of lacosamide in rat plasma. A liquid-liquid
extraction procedure was optimized to extract lacosamide from rat plasma. Chromatographic
separation was accomplished using a reversed phase C18 Hichrom (250×4.6 mm, 5 µm) column with the mobile phase consisting of acetonitrile-phosphate
buffer (pH 3.2±0.1; 20 mM) (21:79, v/v) at a flow rate of 1 mL/min. Both intra- and
inter day assay precision and accuracy were lower than 15% CV. The lower limit of
quantitation was 25 ng/mL for lacosamide and the response was linear in a concentration
range from 25 to 10 000 ng/mL. The developed method was successfully used for the
preclinical pharmacokinetic study of lacosamide in rats.
Key words
lacosamide - HPLC method - rat plasma - pharmacokinetics - validation
