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Synlett 2012; 23(12): 1840-1842
DOI: 10.1055/s-0032-1316554
letter
© Georg Thieme Verlag Stuttgart · New York

Monitoring Biocatalytic Transformations Mediated by Polyketide Synthase Enzymes in Cell Lysate via Fluorine NMR

Shawn K. Piasecki
a   Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A5300, Austin, TX 78712, USA
,
Adrian T. Keatinge-Clay*
a   Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A5300, Austin, TX 78712, USA
b   Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, TX 78712, USA, Fax: +1(512)4718696   eMail: adriankc@mail.utexas.edu
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Publikationsverlauf

Received: 12. April 2012

Accepted after revision: 25. Mai 2012

Publikationsdatum:
04. Juli 2012 (online)

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Abstract

The biocatalytic employment of modular polyketide synthase enzymes in cell lysate has become a viable route to preparative quantities of synthetically valuable polyketide fragments. We report the quantitative, uninvasive, and continuous monitoring of such biocatalytic reactions by observing trifluoromethyl-bearing substrates via 19F NMR spectroscopic analysis. To demonstrate the utility of this technique, we followed reactions catalyzed by a thioesterase and several ketoreductases.

Key words

biosynthesis - biocatalysis - polyketide synthase enzymes - polyketide - fluorine NMR

Supporting Information

    for this article is available online at http://www.thieme-connect.com/ejournals/toc/synlett.
  • Supporting Information
 

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