Download PDF
Planta Med 2013; 79(09): 797-805
DOI: 10.1055/s-0032-1328596
Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

Feng Zeng
1   The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China
2   Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, CAS, Shanghai, China
,
Wei Wang
3   TCM and Ethnomedicine Innovation & Development Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China
,
Shuhong Guan
2   Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, CAS, Shanghai, China
,
Chunru Cheng
2   Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, CAS, Shanghai, China
,
Min Yang
2   Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, CAS, Shanghai, China
,
Bharathi Avula
4   Sino-US TCM Research Center, National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA
,
Ikhlas A. Khan
4   Sino-US TCM Research Center, National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA
,
De-an Guo
1   The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China
2   Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, CAS, Shanghai, China
› Author Affiliations
Further Information

Publication History

received 18 July 2012
revised 17 March 2013

accepted 21 April 2013

Publication Date:
22 May 2013 (online)

    Permissions and Reprints

Abstract

A liquid chromatography–electrospray ionization–mass spectrometry method with multiple reaction monitoring was established for simultaneous quantification of 18 bioactive constituents from the stem and root of Tripterygium wilfordii Hook. f. collected from different places in China and various commercial preparations. The chromatographic separations were achieved on an Agilent Poroshell SB-C18 column (150 × 4.6 mm, 3.5 µm) with gradient elution using acetonitrile and 0.03 % formic acid aqueous solution in 45 min. Detection was performed in the positive ionization mode by monitoring the precursor–product combination. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability, and accuracy. All calibration curves showed good linearity (r > 0.9990) within the test range. The established method showed good precision and accuracy with intraday and interday variations of 2–5 % and 1–4 %, respectively, and recoveries of 95.5–104.5 %.

Key words

Tripterygium wilfordii - sesquiterpenoid alkaloids - diterpenoids - triterpenoids - HPLC-ESI-MS - multiple reaction monitoring - Celastraceae

Supporting Information

 

  • References

  • 1 Bao J, Dai SM. A Chinese herb Tripterygium wilfordii Hook F in the treatment of rheumatoid arthritis: mechanism, efficacy, and safety. Rheumatol Int 2011; 31: 1123-1129
    CrossrefPubMedGoogle Scholar
  • 2 Tao X, Davis LS, Lipsky PE. Effect of an extract of the Chinese herbal remedy Tripterygium wilfordii Hook F on human immune responsiveness. Arthritis Rheum 1991; 34: 1274-1281
    CrossrefPubMedGoogle Scholar
  • 3 Tao X, Davis LS, Hashimoto K, Lipsky PE. The Chinese herbal remedy, T2, inhibits mitogen-induced cytokine gene transcription by T cells, but not initial signal transduction. J Pharmacol Exp Ther 1996; 276: 316-325
    PubMedGoogle Scholar
  • 4 Ma J, Dey M, Yang H, Poulev A, Pouleva R, Dorn R, Lipsky PE, Kennelly EJ, Raskin I. Anti-inflammatory and immunosuppressive compounds from Tripterygium wilfordii . Phytochemistry 2007; 68: 1172-1178
    CrossrefPubMedGoogle Scholar
  • 5 Goldbach-Mansky R, Wilson M, Fleischmann R, Olsen N, Silverfield J, Kempf P, Kivitz A, Sherrer Y, Pucino F, Csako G, Costello R, Pham TH, Snyder C, van der Heijde D, Tao X, Wesley R, Lipsky PE. Comparison of Tripterygium wilfordii Hook F versus sulfasalazine in the treatment of rheumatoid arthritis: a randomized trial. Ann Intern Med 2009; 151: 229-240 W49–W51
    CrossrefPubMedGoogle Scholar
  • 6 Sylvester J, Liacini A, Li WQ, Dehnade F, Zafarullah M. Tripterygium wilfordii Hook F extract suppresses proinflammatory cytokine-induced expression of matrix metalloproteinase genes in articular chondrocytes by inhibiting activating protein-1 and nuclear factor-kappa B activities. Mol Pharmacol 2001; 59: 1196-1205
    PubMedGoogle Scholar
  • 7 Brinker AM, Ma J, Lipsky PE, Raskin I. Medicinal chemistry and pharmacology of genus Tripterygium (Celastraceae). Phytochemistry 2007; 68: 732-766
    CrossrefPubMedGoogle Scholar
  • 8 Gong Y, Xue B, Jiao J, Jing L, Wang X. Triptolide inhibits COX-2 expression and PGE2 release by suppressing the activity of NF-kappaB and JNK in LPS-treated microglia. J Neurochem 2008; 107: 779-788
    CrossrefPubMedGoogle Scholar
  • 9 Kim DH, Shin EK, Kim YH, Lee BW, Jun JG, Park JH, Kim JK. Suppression of inflammatory responses by celastrol, a quinone methide triterpenoid isolated from Celastrus regelii . Eur J Clin Invest 2009; 39: 819-827
    CrossrefPubMedGoogle Scholar
  • 10 Kannaiyan R, Shanmugam MK, Sethi G. Molecular targets of celastrol derived from Thunder of God Vine: potential role in the treatment of inflammatory disorders and cancer. Cancer Lett 2011; 303: 9-20
    CrossrefPubMedGoogle Scholar
  • 11 Chou CT. Alternative therapies: what role do they have in the management of lupus?. Lupus 2010; 19: 1425-1429
    CrossrefPubMedGoogle Scholar
  • 12 Liu Z, Ma L, Zhou GB. The main anticancer bullets of the Chinese medicinal herb, thunder god vine. Molecules 2011; 16: 5283-5297
    CrossrefPubMedGoogle Scholar
  • 13 Lu L, Kanwar J, Schmitt S, Cui QC, Zhang C, Zhao C, Dou QP. Inhibition of tumor cellular proteasome activity by triptolide extracted from the Chinese medicinal plant ʼthunder god vineʼ. Anticancer Res 2011; 31: 1-10
    PubMedGoogle Scholar
  • 14 Kim Y, Kang H, Jang SW, Ko J. Celastrol inhibits breast cancer cell invasion via suppression of NF-ĸB-mediated matrix metalloproteinase-9 expression. Cell Physiol Biochem 2011; 28: 175-184
    CrossrefPubMedGoogle Scholar
  • 15 Zeng F, Wang W, Wu Y, Dey M, Ye M, Avery MA, Khan IA, Guo DA. Two prenylated and C-methylated flavonoids from Tripterygium wilfordii . Planta Med 2010; 76: 1596-1599
    Article in Thieme ConnectPubMedGoogle Scholar
  • 16 Brinker AM, Raskin I. Determination of triptolide in root extracts of Tripterygium wilfordii by solid-phase extraction and reverse-phase high-performance liquid chromatography. J Chromatogr A 2005; 1070: 65-70
    PubMedGoogle Scholar
  • 17 Ma J, Schmidt BM, Poulev A, Raskin I. Determination of tripdiolide in root extracts of Tripterygium wilfordii by solid-phase extraction and reversed-phase high-performance liquid chromatography. Phytochem Anal 2008; 19: 348-352
    CrossrefPubMedGoogle Scholar
  • 18 Luo XL, Shao Q, Qu HB, Cheng YY. Simple method for determination of five terpenoids from different parts of Tripterygium wilfordii and its preparations by HPLC coupled with evaporative light scattering detection. J Sep Sci 2007; 30: 1284-1291
    CrossrefPubMedGoogle Scholar
  • 19 Li K, Wang S. Fingerprint chromatogram analysis of extracts from the leaves of Tripterygium wilfordii Hook. F. by high performance liquid chromatography. J Sep Sci 2005; 28: 653-657
    CrossrefPubMedGoogle Scholar
  • 20 Ouyang XK, Jin MC, He CH. Simultaneous determination of four sesquiterpene alkaloids in Tripterygium wilfordii Hook. F. extracts by high-performance liquid chromatography. Phytochem Anal 2007; 18: 320-325
    CrossrefPubMedGoogle Scholar
  • 21 Ribas-Agustí A, Gratacós-Cubarsí M, Sárraga C, García-Regueiro JA, Castellari M. Analysis of eleven phenolic compounds including novel p-coumaroyl derivatives in lettuce (Lactuca sativa L.) by ultra-high-performance liquid chromatography with photodiode array and mass spectrometry detection. Phytochem Anal 2011; 22: 555-563
    CrossrefPubMedGoogle Scholar
  • 22 Cui Y, Wang Q, Shi X, Zhang X, Sheng X, Zhang L. Simultaneous quantification of 14 bioactive constituents in Forsythia suspensa by liquid chromatography-electrospray ionisation-mass spectrometry. Phytochem Anal 2010; 21: 253-260
    CrossrefPubMedGoogle Scholar
  • 23 Ali I, Gaitonde VD, Grahn A. Halo columns: new generation technology for high speed liquid chromatography. J Chromatogr Sci 2010; 48: 386-394
    CrossrefPubMedGoogle Scholar
  • 24 Rodrigues-Fo E, Barros FA, Fernandes JB, da Silva MF. Studies towards the detection and identification of sesquiterpene pyridine alkaloids in Peritassa campestris by mass spectrometry. Phytochem Anal 2001; 12: 185-193
    CrossrefPubMedGoogle Scholar
  • 25 Zhou Y, He MF, Choi FF, He ZH, Song JZ, Qiao CF, Li SL, Xu HX. A high-sensitivity UPLC-MS/MS method for simultaneous determination and confirmation of triptolide in zebrafish embryos. Biomed Chromatogr 2011; 25: 851-857
    CrossrefPubMedGoogle Scholar
  • 26 Li R, Peng AH, He CM, Wang XH, Shi JX, Chen LJ, Wei YQ. Analysis of triptophenolide and its related compounds from Tripterygium wilfordii Hook.f by electrospray ionization tandem mass spectrometry. Int J Mass Spectrom 2008; 278: 38-49
    PubMedGoogle Scholar