Simultaneous Quantification of 18 Bioactive Constituents in
                        Tripterygium wilfordii Using Liquid Chromatography-Electrospray
                    Ionization-Mass Spectrometry

Feng Zeng; Wei Wang; Shuhong Guan; Chunru Cheng; Min Yang; Bharathi Avula; Ikhlas A. Khan; De-an Guo

doi:10.1055/s-0032-1328596

Planta Medica, Inhaltsverzeichnis

Planta Med 2013; 79(09): 797-805
DOI: 10.1055/s-0032-1328596

Analytical Studies

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

Autor*innen

Feng Zeng

¹The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China

²Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, CAS, Shanghai, China
Wei Wang

³TCM and Ethnomedicine Innovation & Development Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China
Shuhong Guan

²Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, CAS, Shanghai, China
Chunru Cheng

²Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, CAS, Shanghai, China
Min Yang

²Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, CAS, Shanghai, China
Bharathi Avula

⁴Sino-US TCM Research Center, National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA
Ikhlas A. Khan

⁴Sino-US TCM Research Center, National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, MS, USA
De-an Guo

¹The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China

²Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, CAS, Shanghai, China

Abstract

A liquid chromatography–electrospray ionization–mass spectrometry method with multiple reaction monitoring was established for simultaneous quantification of 18 bioactive constituents from the stem and root of Tripterygium wilfordii Hook. f. collected from different places in China and various commercial preparations. The chromatographic separations were achieved on an Agilent Poroshell SB-C₁₈ column (150 × 4.6 mm, 3.5 µm) with gradient elution using acetonitrile and 0.03 % formic acid aqueous solution in 45 min. Detection was performed in the positive ionization mode by monitoring the precursor–product combination. The validation of the method included tests of linearity, sensitivity, precision, repeatability, stability, and accuracy. All calibration curves showed good linearity (r > 0.9990) within the test range. The established method showed good precision and accuracy with intraday and interday variations of 2–5 % and 1–4 %, respectively, and recoveries of 95.5–104.5 %.

Key words

Tripterygium wilfordii - sesquiterpenoid alkaloids - diterpenoids - triterpenoids - HPLC-ESI-MS - multiple reaction monitoring - Celastraceae

Volltext

Referenzen

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