Drug Res (Stuttg) 2013; 63(03): 109-120
DOI: 10.1055/s-0032-1331765
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Molecular Design, Synthesis and Cell Based HCV Replicon Assay of Novel Benzoxazole Derivatives

M. A. H. Ismail
1   Pharmaceutical Chemistry Department, Faculty of Pharmacy Ain Shams University, Cairo, Egypt
M. Adel
1   Pharmaceutical Chemistry Department, Faculty of Pharmacy Ain Shams University, Cairo, Egypt
N.S. M. Ismail
1   Pharmaceutical Chemistry Department, Faculty of Pharmacy Ain Shams University, Cairo, Egypt
K.A. M. Abouzid
1   Pharmaceutical Chemistry Department, Faculty of Pharmacy Ain Shams University, Cairo, Egypt
› Author Affiliations
Further Information

Publication History

received 28 June 2012

accepted 05 December 2012

Publication Date:
26 February 2013 (online)


Hepatitis C virus inhibitors based on benzoxazole scaffold were designed based on molecular modeling simulation study including docking into the NS5B polymerase active site. Several compounds showed significant high simulation docking scores relative to the assigned benzimidazole lead compound. The designed compounds were synthesized, structurally elucidated and their antiviral activity was evaluated through cell-based replicon in cultured Huh 5-2 cells. A number of the synthesized compounds showed significant inhibitory activity ranging from (52.2% inhibition up to 98% at<50 µg/mL). N-Benzyl-2-phenylbenzo[1,3]oxazole-5-carboxamide (8b) and N-Phenethyl-2-phenylbenzo[1,3] oxazole-5-carboxamide (8c) demonstrated genuine HCV inhibitory activity with EC50 values of 41.6 and 24.5 µg/mL respectively.

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