Activated Translation Signaling in Placenta from Pregnant Women with Gestational Diabetes Mellitus: Possible Role of Leptin

A. Pérez-Pérez; J. L. Maymó; Y. P. Gambino; P. Guadix; J. L. Dueñas; C. L. Varone; V. Sánchez-Margalet

doi:10.1055/s-0032-1333276

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2013; 45(06): 436-442
DOI: 10.1055/s-0032-1333276

Original Basic

Activated Translation Signaling in Placenta from Pregnant Women with Gestational Diabetes Mellitus: Possible Role of Leptin

Authors

A. Pérez-Pérez

¹Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain
J. L. Maymó

²Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Y. P. Gambino

²Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
P. Guadix

³Servicio de Ginecología y Obstetricia, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain
J. L. Dueñas

³Servicio de Ginecología y Obstetricia, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain
C. L. Varone

²Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
V. Sánchez-Margalet

¹Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain

Abstract

Placentas from gestational diabetes (GDM) suffer from structural and functional changes including overgrowth. That is why we aimed to study [³H]-leucine incorporation into protein in addition to translation signaling in placenta from GDM. Thus, we investigated the expression of leptin and leptin receptor (LEPR), as well as the activation state of signaling proteins regulating protein synthesis, such as mTOR, S6 Kinase, EIF4E-BP1, EIF4E, and eEF2 by measuring protein phosphorylation by immunoblot. [³H]-Leucine incorporation into protein also was determined in trophoblastic placenta explants from GDM and control pregnancy. We found that leptin and LEPR expression are increased in placentas from GDM and the translation machinery activity as well as [³H]-leucine incorporation into protein were higher in placentas from GDM compared with placentas from control pregnancy. In conclusion, protein synthesis rate is increased in placenta from GDM patients, and this may be due, at least in part, by the activation of translation signaling. The increased expression of leptin and LEPR may contribute to these effects. These results may provide a possible mechanism for the previously observed increase in placenta growth in GDM.

Key words

leptin - LEPR - trophoblastic cells - mTOR - EIF4EBP1 - translation - gestational diabetes

Full Text

References

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