Characterization of the Specificity of Imidazoline I-1 Receptor Antibody for Subtype of Imidazoline Receptors In Vitro

L.-Y. Wang; P.-M. Ku; S.-H. Chen; L.-J. Chen; Y.-M. Yu; J.-T. Cheng

doi:10.1055/s-0033-1333765

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2013; 45(07): 485-489
DOI: 10.1055/s-0033-1333765

Original Basic

Characterization of the Specificity of Imidazoline I-1 Receptor Antibody for Subtype of Imidazoline Receptors In Vitro

Autor*innen

L.-Y. Wang

¹Department of Pediatrics, Department of Medical Research, Chi-Mei Medical Center, Yong Kang, Tainan City, Taiwan

²The Center of General Education, Chia Nan University of Pharmacy & Science, Rende Dist, Tainan City, Taiwan
P.-M. Ku

³Department of Cardiology, Department of Medical Research, Chi-Mei Medical Center, Yong Kang, Tainan City, Taiwan
S.-H. Chen

⁴Department of Obstetrics and Gynecology, Department of Medical Research, Chi-Mei Medical Center, Yong Kang, Tainan City, Taiwan

⁵Department of Medical Research, Chi-Mei Medical Center, Yong Kang, Tainan City, Taiwan
L.-J. Chen

⁶Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan City, Taiwan
Y.-M. Yu

⁷Department of Nutrition and Institute of Medical Science, College of Health Science, Chang Jung Christian University, Guei-Ren, Tainan City, Taiwan
J.-T. Cheng

⁵Department of Medical Research, Chi-Mei Medical Center, Yong Kang, Tainan City, Taiwan

⁶Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan City, Taiwan

Abstract

Specific antibodies are essential in the study of receptor protein. Gene matching shows that Nischarin (NISCH) is a mouse homologue of human imidazoline receptor antisera-selective (IRAS) protein, a viable candidate for imidazoline I-1 receptor. However, selectivity of this antibody against imidazoline I-2 or imidazoline I-3 receptors remained obscure. At first, an intracerebroventricular (ICV) injection of anti-NISCH antibody blocked the blood pressure lowering action of rilmenidine (I-1 receptor agonist) in spontaneous hypertensive rat (SHR). However, the same injection of anti-NISCH antibody showed no effect in SHR treated with clonidine (α₂ agonist). In order to clarify the selectivity of anti-NISCH antibody for each subtype of imidazoline receptors, this anti-NISCH antibody was subjected to the lysate of organs isolated from Wistar rats including cortex, hippocampus, cerebellum, and brain stem as central nervous tissues, and heart, liver, pancreas, skeletal muscle, kidney, prostate, and bladder as peripheral tissues. The results show that anti-NISCH antibody positively reacted with all tissues including heart, pancreas, skeletal muscle, kidney and bladder by Western blot analysis. Also, the blotting spots for anti-NISCH antibody show a concentration-dependent manner. Moreover, anti-NISCH antibody blocked the action of glucose uptake induced by 2-BFI (I-2 receptor agonist) in L6 cells. Taken together, the obtained data suggest that anti-NISCH antibody can be used not only for imidazoline I-1 receptor but also for I-2 and I-3 subtypes in immunoassays.

Key words

imidazoline I-1 receptor - imidazoline I-2 receptor - imidazoline I-3 receptor - Nischarin (HISCH) - imidazoline receptor antisera-selective (IRAS) protein

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Referenzen

References
1 Bousquet P, Feldman J, Schwartz J. Central cardiovascular effects of alpha adrenergic drugs: differences between catecholamines and imidazolines. J Pharmacol Exp Ther 1984; 230: 232-236
2 Michel MC, Insel PA. Are there multiple imidazoline binding sites?. Trends Pharmacol Sci 1989; 10: 342-344
3 Chan SL, Brown CA, Scarpello KE, Morgan NG. The imidazoline site involved in control of insulin secretion: characteristics that distinguish it from I1- and I2-sites. Br J Pharmacol 1994; 112: 1065-1070
4 Morgan NG, Chan SL, Mourtada M, Monks LK, Ramsden CA. Imidazolines and pancreatic hormone secretion. Ann N Y Acad Sci 1999; 881: 217-228
5 Mayer G, Taberner PV. Effects of the imidazoline ligands efaroxan and KU14R on blood glucose homeostasis in the mouse. Eur J Pharmacol 2002; 454: 95-102
6 Escriba PV, Ozaita A, Garcia-Sevilla JA. Pharmacologic characterization of imidazoline receptor proteins identified by immunologic techniques and other methods. Ann N Y Acad Sci 1999; 881: 8-25
7 Dontenwill M, Vonthron C, Greney H, Magnier C, Heemskerk F, Bousquet P. Identification of human I1 receptors and their relationship to alpha 2-adrenoceptors. Ann N Y Acad Sci 1999; 881: 123-134
8 Heemskerk FM, Dontenwill M, Greney H, Vonthron C, Bousquet P. Evidence for the existence of imidazoline-specific binding sites in synaptosomal plasma membranes of the bovine brainstem. J Neurochem 1998; 71: 2193-2202
9 Tesson F, Parini A. Identification of an imidazoline-guanidinium receptive site in mitochondria from rabbit cerebral cortex. Eur J Pharmacol 1991; 208: 81-83
10 Tesson F, Limon I, Parini A. Tissue-specific localization of mitochondrial imidazoline-guanidinium receptive sites. Eur J Pharmacol 1992; 219: 335-338
11 Martin-Gomez JI, Ruiz J, Callado LF, Garibi JM, Aguinaco L, Barturen F, Javier Meana J. Increased density of I2-imidazoline receptors in human glioblastomas. Neuroreport 1996; 7: 1393-1396
12 Tesson F, Limon-Boulez I, Urban P, Puype M, Vandekerckhove J, Coupry I, Pompon D, Parini A. Localization of I2-imidazoline binding sites on monoamine oxidases. J Biol Chem 1995; 270: 9856-9861
13 Ulibarri I, Soto J, Ruiz J, Ballesteros J, Jauregui JV, Meana JJ. I2-imidazoline receptors in platelets of patients with Parkinson’s disease and Alzheimer’s type dementia. Ann N Y Acad Sci 1999; 881: 199-202
14 Raasch W, Muhle H, Dominiak P. Modulation of MAO activity by imidazoline and guanidine derivatives. Ann N Y Acad Sci 1999; 881: 313-331
15 Prince JT, Milona N, Stallcup WB. Characterization of a partial cDNA clone for the NILE glycoprotein and identification of the encoded polypeptide domain. J Neurosci 1989; 9: 1825-1834
16 Piletz JE, Ivanov TR, Sharp JD, Ernsberger P, Chang CH, Pickard RT, Gold G, Roth B, Zhu H, Jones JC, Baldwin J, Reis DJ. Imidazoline receptor antisera-selected (IRAS) cDNA: cloning and characterization. DNA Cell Biol 2000; 19: 319-329
17 Alahari SK, Lee JW, Juliano RL. Nischarin, a novel protein that interacts with the integrin alpha5 subunit and inhibits cell migration. J Cell Biol 2000; 151: 1141-1154
18 Zhang J, Abdel-Rahman AA. Nischarin as a functional imidazoline (I1) receptor. FEBS Lett 2006; 580: 3070-3074
19 Obregon D, Hou H, Deng J, Giunta B, Tian J, Darlington D, Shahaduzzaman M, Zhu Y, Mori T, Mattson MP, Tan J. Soluble amyloid precursor protein-alpha modulates beta-secretase activity and amyloid-beta generation. Nature Commun 2012; 3: 777
20 Zhu QM, Lesnick JD, Jasper JR, MacLennan SJ, Dillon MP, Eglen RM, Blue Jr DR. Cardiovascular effects of rilmenidine, moxonidine and clonidine in conscious wild-type and D79N alpha2A-adrenoceptor transgenic mice. Br J Pharmacol 1999; 126: 1522-1530
21 Yang TT, Liu IM, Wu HT, Cheng JT. Mediation of protein kinase C zeta in mu-opioid receptor activation for increase of glucose uptake into cultured myoblast C2C12 cells. Neurosci Lett 2009; 465: 177-180
22 Alonso-Castro AJ, Zapata-Bustos R, Dominguez F, Garcia-Carranca A, Salazar-Olivo LA. Magnolia dealbata Zucc and its active principles honokiol and magnolol stimulate glucose uptake in murine and human adipocytes using the insulin-signaling pathway. Phytomedicine 2011; 18: 926-933
23 Alonso-Castro AJ, Salazar-Olivo LA. The anti-diabetic properties of Guazuma ulmifolia Lam are mediated by the stimulation of glucose uptake in normal and diabetic adipocytes without inducing adipogenesis. J Ethnopharmacol 2008; 118: 252-256
24 Yang TT, Ku PM, Hsu CT, Chung HH, Lee WJ, Cheng JT. Mediation of AMP Kinase in the Increase of Glucose Uptake in L6 Cells Induced by Activation of Imidazoline I-2 Receptors. Horm Metab Res 2012; Dec 7 [Epub ahead or print]
25 Bhaskar JJ, Salimath PV, Nandini CD. Stimulation of glucose uptake by Musa sp. (cv. elakki bale) flower and pseudostem extracts in Ehrlich ascites tumor cells. J Sci Food Agric 2011; 91: 1482-1487
26 Piletz JE, Wang G, Zhu H. Cell signaling by imidazoline-1 receptor candidate, IRAS, and the nischarin homologue. Ann N Y Acad Sci 2003; 1009: 392-399
27 Zhang J, Abdel-Rahman AA. Inhibition of nischarin expression attenuates rilmenidine-evoked hypotension and phosphorylated extracellular signal-regulated kinase 1/2 production in the rostral ventrolateral medulla of rats. J Pharmacol Exp Ther 2008; 324: 72-78
28 Sano H, Liu SC, Lane WS, Piletz JE, Lienhard GE. Insulin receptor substrate 4 associates with the protein IRAS. J Biol Chem 2002; 277: 19439-19447
29 Sun Z, Chang CH, Ernsberger P. Identification of IRAS/Nischarin as an I1-imidazoline receptor in PC12 rat pheochromocytoma cells. J Neurochem 2007; 101: 99-108
30 Li F, Wu N, Su R, Chen Y, Lu X, Liu Y, Li J. Imidazoline receptor antisera-selected/Nischarin regulates the effect of agmatine on the development of morphine dependence. Addiction Biol 2012; 17: 392-408
31 Zhang J, Abdel-Rahman AA. Inhibition of nischarin expression attenuates rilmenidine-evoked hypotension and phosphorylated extracellular signal-regulated kinase 1/2 production in the rostral ventrolateral medulla of rats. J Pharmacol Exp Therap 2008; 324: 72-78
32 Baranwal S, Wang Y, Rathinam R, Lee J, Jin L, McGoey R, Pylayeva Y, Giancotti F, Blobe GC, Alahari SK. Molecular characterization of the tumor-suppressive function of nischarin in breast cancer. J Nat Cancer Inst 2011; 103: 1513-1528
33 Wang B, Liu Y, Shan Y, Yao Z, Liu X, Su R, Sun Q, Cong Y, Li J. Generation and characterization of novel human IRAS monoclonal antibodies. J Biomed Biotechnol 2009; 2009: 973754
34 Gongadze NV, Antelava NA, Kezeli TD. Imidazoline receptors. Georgian Med News 2008; 44-47
35 Nechifor M, Ciubotariu D. Behavioral involvement of imidazoline system. Revista Med-Chirurg Soc Med Nat Iasi 2012; 116: 552-556
36 Kawamura K, Yui J, Konno F, Yamasaki T, Hatori A, Wakizaka H, Fujinaga M, Kumata K, Yoshida Y, Ogawa M, Nengaki N, Yanamoto K, Fukumura T, Zhang MR. Synthesis and evaluation of PET probes for the imaging of I2 imidazoline receptors in peripheral tissues. Nucl Med Biol 2012; 39: 89-99
37 Morgan NG, Chan SL. Imidazoline binding sites in the endocrine pancreas: can they fulfil their potential as targets for the development of new insulin secretagogues?. Curr Pharmaceut Design 2001; 7: 1413-1431
38 Malloy BJ, Price DT, Price RR, Bienstock AM, Dole MK, Funk BL, Rudner XL, Richardson CD, Donatucci CF, Schwinn DA. Alpha1-adrenergic receptor subtypes in human detrusor. J Urol 1998; 160: 937-943
39 Hosoda C, Koshimizu TA, Tanoue A, Nasa Y, Oikawa R, Tomabechi T, Fukuda S, Shinoura H, Oshikawa S, Takeo S, Kitamura T, Cotecchia S, Tsujimoto G. Two alpha1-adrenergic receptor subtypes regulating the vasopressor response have differential roles in blood pressure regulation. Mol Pharmacol 2005; 67: 912-922
40 Devic E, Xiang Y, Gould D, Kobilka B. Beta-adrenergic receptor subtype-specific signaling in cardiac myocytes from beta(1) and beta(2) adrenoceptor knockout mice. Mol Pharmacol 2001; 60: 577-583
41 Spitzer N, Edwards DH, Baro DJ. Conservation of structure, signaling and pharmacology between two serotonin receptor subtypes from decapod crustaceans, Panulirus interruptus and Procambarus clarkii. J Exp Biol 2008; 211: 92-105