Drug Res (Stuttg) 2013; 63(04): 185-191
DOI: 10.1055/s-0033-1334881
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Taurine-magnesium Coordination Compound Attenuates Hypoxia/Reoxygenation Induced Ion Channel Dysfunction in Rat Ventricular Myocytes

L. Zhao
1   Department of Pharmacology, Tianjin Medical University, Tianjin, China
,
J.-S. Lou
1   Department of Pharmacology, Tianjin Medical University, Tianjin, China
,
Y. Kang
1   Department of Pharmacology, Tianjin Medical University, Tianjin, China
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 17. August 2012

accepted 28. Januar 2013

Publikationsdatum:
13. März 2013 (online)

Abstract

Because of the known anti-arrhythmic effects of taurine-magnesium coordination compound (TMCC), the aim of the present study was to explore the electrophysiological effects of TMCC on hypoxia/reoxygenation (H/R)-induced arrhythmias in rat ventricular myocytes. Sodium current (I Na), the L-type calcium current (I Ca,L), and the transient outward potassium current (I to) were evaluated using whole-cell patch-clamp recordings in rat ventricular myocytes following H/R injury. The H/R group significantly decreased sodium currents, while L-type calcium currents and transient outward potassium currents was significantly increased (all p<0.01). TMCC (200 and 400 μM) prevented abnormal sodium currents induced by H/R by inhibiting steady-state inactivation. It also counteracted abnormal L-type calcium currents induced by H/R by inhibiting steady-state activation and facilitating steady-state inactivation. In addition, it mitigated abnormal transient outward potassium currents induced by H/R by inhibiting steady-state activation. TMCC prevents H/R-induced arrhythmias in rat ventricular myocytes by modifying ion channel function.

 
  • References

  • 1 Hanna J, Chahine R, Aftimos G et al. Protective effect of taurine against free radicals damage in the rat myocardium. Exp Toxicol Pathol 2004; 56: 189-194
  • 2 Coates BJ, Broderick TL, Batia LM et al. MgSO4 prevents left ventricular dysfunction in an animal model of preeclampsia. Am J Obstet Gynecol 2006; 195: 1398-1403
  • 3 Standley CA, Batia L, Yueh G. Magnesium sulfate effectively reduces blood pressure in an animal model of preeclampsia. J Matern Fetal Neonatal Med 2006; 19: 171-176
  • 4 Lee NY, Kang YS. Regulation of taurine transport at the blood-placental barrier by calcium ion, PKC activator and oxidative stress conditions. J Biomed Sci 2010; 17: S37
  • 5 Kang YS. Downregulation of taurine transport by calcium blockers in osteoblast cells. Ady Exp Med Biol 2009; 643: 513-521
  • 6 Beyranvand MR, Khalafi MK, Roshan VD et al. Effect of taurine supplementation on exercise capacity of patients with heart failure. J Cardiol 2011; 57: 333-337
  • 7 Bkaily G, Jaalouk D, Sader S et al. Taurine indirectly increases Ca]i by inducing Ca2+ influx through the Na(+)-Ca2+ exchanger. Mol Cell Biochem 1998; 188: 187-197
  • 8 Shimosawa T, Fujita T. Magnesium and N-type calcium channel. Clin Calcium 2005; 15: 239-244
  • 9 Liu H, Chen H, Yang X. Protective effect of magnesium on ionic channels in guinea pig ventricular myocytes during hypoxia. Zhonghua Yi Xue Za Zhi 1997; 77: 505-508
  • 10 Yin Y, Wen K, Wu Y et al. Inhibition of Sodium Current by Taurine Magnesium Coordination Compound Prevents Cesium Chloride-Induced Arrhythmias. Biol Trace Elem Res 2012; 146: 192-198
  • 11 Zhao L, Lou J, Wu H et al. Effects of Taurine-Magnesium Coordination Compound on Ionic Channels in Rat Ventricular Myocytes of Arrhythmia Induced by Ouabain. Biol Trace Elem Res 2012; 147: 275-284
  • 12 Li Q, Li Z, Mei Y et al. Neuregulin attenuated cerebral ischemia-reperfusion injury via inhibiting apoptosis and upregulating aquaporin-4. Neurosci Lett 2008; 443: 155-159
  • 13 Li C, Jackson RM. Reactive species mechanisms of cellular hypoxia-reoxygenation injury. Am J Physiol Cell Physiol 2002; 282: C227-C241
  • 14 Platoshyn O, Yu Y, Golovina VA et al. Chronic hypoxia decreases K(V) channel expression and function in pulmonary artery myocytes. Am J Physiol Lung Cell Mol Physiol 2001; 280: L801-L812
  • 15 Fearon IM, Palmer AC, Balmforth AJ et al. Modulation of recombinant human cardiac L-type Ca2+ channel alpha1C subunits by redox agents and hypoxia. J Physiol 1999; 514 (Pt 3) 629-637
  • 16 Wang YX, Dhulipala PK, Kotlikoff MI. Hypoxia inhibits the Na(+)/Ca(2+) exchanger in pulmonary artery smooth muscle cells. FASEB J 2000; 14: 1731-1740
  • 17 Rowe GT, Manson NH, Caplan M et al. Hydrogen peroxide and hydroxyl radical mediation of activated leukocyte depression of cardiac sarcoplasmic reticulum. Participation of the cyclooxygenase pathway. Circ Res 1983; 53: 584-591
  • 18 Lin MJ, Leung GP, Zhang WM et al. Chronic hypoxia-induced upregulation of store-operated and receptor-operated Ca2+ channels in pulmonary arterial smooth muscle cells: a novel mechanism of hypoxic pulmonary hypertension. Circ Res 2004; 95: 496-505
  • 19 Kawakami M, Okabe E. Superoxide anion radical-triggered Ca2+ release from cardiac sarcoplasmic reticulum through ryanodine receptor Ca2+ channel. Mol Pharmacol 1998; 53: 497-503
  • 20 Zhou SS, Yang J, Li YQ et al. Effect of Cl- channel blockers on aconitine-induced arrhythmias in rat heart. Exp Physiol 2005; 90: 865-872
  • 21 Faber GM, Silva J, Livshitz L et al. Kinetic properties of the cardiac L-type Ca2+ channel and its role in myocyte electrophysiology: a theoretical investigation. Biophys J 2007; 92: 1522-1543
  • 22 Chen W, Wasserstrom JA, Shiferaw Y. Role of coupled gating between cardiac ryanodine receptors in the genesis of triggered arrhythmias. Am J Physiol Heart Circ Physiol 2009; 297: H171-H180
  • 23 Triggle DJ. Calcium channel antagonists: clinical uses – past, present and future. Biochem Pharmacol 2007; 74: 1-9
  • 24 Niwa N, Nerbonne JM. Molecular determinants of cardiac transient outward potassium current (I(to)) expression and regulation. J Mol Cell Cardiol 2010; 48: 12-25
  • 25 Monsuez JJ. Cardiac potassium currents and channels. Part II: Implications for clinical practice and therapy. Int J Cardiol 1997; 62: 1-12
  • 26 Di Diego JM, Antzelevitch C. High [Ca2+]o-induced electrical heterogeneity and extrasystolic activity in isolated canine ventricular epicardium. Phase 2 reentry. Circulation 1994; 89: 1839-1850
  • 27 Yao JA, Jiang M, Fan JS et al. Heterogeneous changes in K currents in rat ventricles three days after myocardial infarction. Cardiovasc Res 1999; 44: 132-145
  • 28 Roden DM, Balser JR, George Jr AL et al. Cardiac ion channels. Annu Rev Physiol 2002; 64: 431-475