Stimulatory Effect on Osteoblast Differentiation of Compounds from Cissus quadrangularis L
Cissus quadrangularis L. (CQ), a medicinal plant, is widely used in traditional medicine in Southeast Asia . A number of studies reported health benefits of this plant while many CQ products have been sold as dietary supplements . However, the chemical standardization of CQ pharmacologically active compound has never been indicated. Our previous investigation found that the CQ hexane extract possessed antiosteoporotic effect in ovariectomized mice model . The hexane extract was further isolated using bioassay-guided purification by bone cell culture technique. Five of the 31 sub-fractions stimulated the differentiation process of MC3T3-E1 osteoblast-like cells. The pharmacologically active compounds contained in these sub-fractions were isolated and elucidated. These active compounds will be useful for standardization of both raw material and CQ products. Acknowledgements: This project is financially supported by the Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0201/2549) and the Chao Phraya Abhaibhubejhr Hospital Foundation. References:  Williamson EM (2002) Major herbs of Ayuraveda. Churchill Livingstone. London.  Stohs SJ, Ray SD (2012) Phytother Res. doi: 10.1002/ptr.4846.  Pathomwichaiwat T, et al. (2012) Asian Biomed 6: 377 – 384.