ZWR - Das Deutsche Zahnärzteblatt 2013; 122(01/02): 18-24
DOI: 10.1055/s-0033-1337898
Wissenschaft
Parodontologie
© Georg Thieme Verlag Stuttgart · New York

Die Wirkung von Pflanzenstoffen auf parodontalpathogene Bakterien

The Effect of Plant Extracts on Periodontopathogenic Bacteria
H Staudte
,
B W Sigusch
Further Information

Publication History

Publication Date:
19 February 2013 (online)

Das Ziel der vorliegenden Studie war es, die Wirkung verschiedener Pflanzenextrakte auf das Wachstum parodontalpathogener Bakterien zu untersuchen. Zur Bestimmung des antibakteriellen Effekts von Ingwer (Zingiber officinalis), Salbei (Salvia officinalis), Kamille (Chamomilla recutita), Rosmarin (Rosmarinus officinalis), Nelke (Syzygium aromaticum), Myrrhe (Commiphora abyssinica) und Rathania (Krameria lappacea) gegenüber Fusobacterium nucleatum ATCC 10 953, Porphyromonas gingivalis ATCC 33 277, Actinobacter actinomycetemcomitans ATCC 33 384 und Enterococcus faecalis ATCC 29 212 wurde der Agar-Diffusionstest verwendet. Das Bakterienwachstum konnte durch die meisten Pflanzenextrakte in unterschiedlichem Maße gehemmt werden. Der Nelkenextrakt wies die höchste antibakterielle Wirksamkeit auf, welche teilweise die von Chlorhexidin (Positivkontrolle) übertraf. Die vorliegenden Ergebnisse zeigen, dass Pflanzenstoffe die antibakterielle Parodontitis-Therapie sinnvoll unterstützen könnten.

The aim of this study was to evaluate the inhibition activity of different plant extracts on periodonto-pathogenic bacteria. Supercritical CO2-extracts from Zingiber officinalis (ginger), Salvia officinalis (sage), Chamomilla recutita (camomile), Rosmarinus officinalis (rosemary), Syzygium aromaticum (clove), Krameria lappacea (rathania), and Commiphora abyssinica (myrrh) were tested against Fusobacterium nucleatum ATCC 10 953, Porphyromonas gingivalis ATCC 33 277, Aggregatibacter actinomycetemcomitans ATCC 33 384, and Enterococcus faecalis ATCC 29 212. The antimicrobial activity was determined by the agar diffusions method and the zones of growth inhibition were measured. The bacterial growth was inhibited through the most plant extracts in different extent. The clove extract showed the highest antibacterial effects, which increased the effect of CHX (positive control) partially. The results suggest that the application of special plant extracts may be helpful as adjunct treatment to inactivate periodonto-pathogenic bacteria.

 
  • Literatur

  • 1 Akalin FA, Baltacioglu E, Sengün D et al. A comparative evaluation of the clinical effects of systemic and local doxycycline in the treatment of chronic periodontitis. J Oral Sci 2004; 46: 25-35
  • 2 Barnett M. Role of therapeutic antimicrobial mouthrinses in clinical practice. J Am Dent Res 2003; 134: 699-702
  • 3 Bernardes WA, Lucarini R, Tozatti MG et al. Antibacterial activity of the essential oil from Rosmarinus officinalis and its major components against oral pathogens. Z Naturforsch C 2010; 65: 588-593
  • 4 Borges FM, de Melo MA, Lima JP et al. Antimicrobial effect of chlorhexidine digluconate in dentin: in vitro and in situ study. J Conserv Dent 2012; 15: 22-26
  • 5 Burt S. Essential oils: their antibacterial properties and potential applications in foods – a review. Int J Food Microbiol 2004; 94: 233-253
  • 6 Cai L, Wu CD. Compounds from Syzygium aromaticum possessing growth inhibitory activity against oral pathogens. J Nat Prod 1996; 59: 987-990
  • 7 Ceschel GC, Maffei P, Moretti MD et al. In vitro permeation through porcine buccal mucosa of Salvia desoleana Atzei & Picci essential oil from topical formulations. Int J Pharm 2000; 195: 171-177
  • 8 Chaieb K, Hajlaoui H, Zmantar T et al. The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzygium aromaticum L. Myrtaceae): a short review. Phytother Res 2007; 21: 501-506
  • 9 Chang CP, Chang JY, Wang FY et al. The effect of Chinese medicinal herb Zingiberis rhizoma extract on cytokine secretion by human peripheral blood mononuclear cells. J Ethnopharmacol 1995; 48: 13-19
  • 10 Chaudhari LK, Jawale BA, Sharma S et al. Antimicrobial activity of commercially available essential oil against Streptococcus mutans. J Contemp Dent Pract 2012; 13: 71-74
  • 11 Cox SD, Mann CM, Markham JL et al. The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tee trea oil). J Appl Microbiol 2000; 88: 170-175
  • 12 Devi KP, Nisha SA, Sakthivel R et al. Eugenol (an essential oil) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. J Ethnopharmacol 2010; 130: 107-115
  • 13 Eick S, Goltz S, Nietzsche S et al. Efficiacy of chlorhexidine digluconate-containing formulations and other mouthrinses against periodontopathogenic microorganisms. Quintessence Int 2011; 42: 687-700
  • 14 Feres M, Figueiredo LC, Barreto IM et al. In vitro antimicrobial activity of plant extracts and propolis in saliva samples of healthy and periodontally-involved subjects. J Int Acad Periodontol 2005; 7: 90-96
  • 15 Ficker C, Smith ML, Akpagana K et al. Bioassay-guided isolation and identification of antifungal compounds from ginger. Phytother Res 2003; 17: 897-902
  • 16 Gupta C, Kumari A, Garq AP et al. Comparative study of cinnamon oil and clove oil on some oral microbiota. Acta Biomed 2011; 82: 197-199
  • 17 Hemaiswarya S, Doble M. Synergistic interaction of eugenol with antibiotics against Gram-negative bacteria. Phytomedicine 2009; 16: 997-1005
  • 18 Holder IA, Boyce ST. Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture. Burns 1994; 20: 426-42
  • 19 Liippo J, Kousa P, Lammintausta K. The relevance of chlorhexidine contact allergy. Contact Dermatitis 2011; 64: 229-234
  • 20 Mahady GB, Pendland SL, Yun GS et al. Ginger (Zingiber officinale Roscoe) and the gingerols inhibit the growth of Cag A+ strains of Helicobacter pylori. Anticancer Res 2003; 23: 3699-3702
  • 21 Manju V, Nalini N. Effect of ginger on bacterial enzymes in 1,2-dimethylhydrazine induced experimental colon carcinogenesis. Eur J Cancer Prev 2006; 15: 377-383
  • 22 Moon SE, Kim HY, Cha JD. Synergistic effect between clove oil and its major compounds and antibiotics against oral bacteria. Arch Oral Biol 2011; 56: 907-916
  • 23 Park M, Bae J, Lee DS. Antibacterial activity of [10]-Gingerol and [12]-Gingerol isolated from ginger rhizome against periodontal bacteria. Phytother Res 2008; 22: 1446-1449
  • 24 Pitten FA, Kramer A. Antimicrobial efficacy of antiseptic mouthrinse solutions. Eur J Clin Pharmacol 1999; 55: 95-100
  • 25 Primosch RE, Ahmadi A, Setzer B et al. A retrospective assessment of zinc oxide-eugenol pulpectomies in vital maxillary primary incisors successfully restored with composite resin crowns. Pediatr Dent 2005; 27: 470-477
  • 26 Rahim ZHA, Khan HBSG. Comparative studies on the effect of crude aqueous (CA) and solvent (CM) extracts of clove on the cariogenic properties of Streptococcus mutans. J Oral Sci 2006; 48: 117-123
  • 27 Shapiro S, Meier A, Guggenheim B. The antimicrobial activity of essential oils and essential oil components towards oral bacteria. Oral Microbiol Immunol 1994; 9: 202-208
  • 28 Smullen J, Finney M, Storey DM et al. Prevention of artificial plaque formation in vitro by plant extracts. J Appl Microbiol 2012; 113: 964-973
  • 29 de Souza LB, de Aquino SG, de Souza PP et al. Cytotoxic effects of different concentrations of chlorhexidine. Am J Dent 2007; 20: 400-404
  • 30 Staudte H, Sigusch BW. Die Zahnheilkunde der Indianer – Wurzelzahnbürsten, Solezahnpaste und Pflanzenkaugummis. ZWR – Das deutsche Zahnärzteblatt 2007; 116: 109-116
  • 31 Tozzi R, Mulinacci N, Storlikken K et al. Supercritical extraction of carotenoids from Rosa canina L. hips and their formulation with beta-cyclodextrin. AAPS PharmSciTech 2008; 9: 693-700
  • 32 Xu Y, Wei W. A comparative study of systemic subantimicrobial and topical treatment of minocycline in experimental periodontitis of rats. Arch Oral Biol 2006; 51: 794-803