Planta Med 2013; 79 - PI17
DOI: 10.1055/s-0033-1352107

Phytochemical study of the polar extracts of Cenostigma macrophyllum Tul. (Leguminosae)

HN Brandão 1, CQ Alves 2, JM David 3, JP David 4
  • 1Universidade Estadual de Feira de Santana, Departamento de Saúde, Feira de Santana – Bahia (44036900), Brasil
  • 2Universidade Estadual de Feira de Santana, Departamento de Ciências Exatas, Feira de Santana – Bahia (44036900), Brasil
  • 3Universidade Federal da Bahia, Instituo de Quimica, Salvador – BA (40170290), Brasil
  • 4Universidade Federal da Bahia, Faculdade de Farmacia, Salvador – BA (40170290), Brasil

Cenostigma macrophyllum Tul. (Leguminosae) is a plant popularly known as “canela-de-velho,” “caneleiro” and “catingueira” that can be found in the Brazilian “cerrado” and “caatinga” regions1,2. The leaves, stem barks and flowers of species of this genus are also used as folk medicines to treat stomach and intestinal disorders3. The aqueous leaf extract of C. macrophyllum showed anti-ulcer activity, and the ethanol extract presented antimicrobial, anti-inflammatory and antinociceptive activities4. In previous works we presented the isolation and identification of several substances with biological activity such as bergenin (antinociceptive activity), quercetin, gallic acid, ellagic acid (antioxidant activity), aurentiamide and aurentiamide acetate (anticholinesterase activity)5,6. This work describes the phytochemical study of the leaves of C. macrophyllum. The MeOH extract was partitioned between a solution of MeOH:H2O (9:1) and CH2Cl2, followed by a partition with EtOAc. The EtOAc extract was submitted to a column chromatography that led to isolation and identification of the flavonoids Vitexin, Isovitexin, (2''-O-galloyl) Isovitexin and Nicotiflorin. The structures of the isolated compounds were elucidated by 1H and 13C NMR (uni and bidimensional), IR and MS, beyond comparison with literature dates.

Acknowledgements: We wish to thank CNPq and FAPESB/PRONEM (Brazil) for financial support.

References:

[1] da Silva, M. F. Editora da Universidade Federal do Amazonas: Manaus, 2004.

[2] Queiroz, L. P. Universidade Estadual de Feira de Santana: Feira de Santana, 2009.

[3] Silva, H. R. et al. (2007) Química Nova, 30: 1877.

[4] Sousa, C. M. M. et al. (2007) Química Nova, 30: 351.

[5] Alves, C. Q. et al. (2012), Química Nova, 35: 1137 – 1140.

[6] Alves, C. Q. et al. (2013), Parmaceutical Biology, (doi:10.3109/13880209.2013.770536).