Planta Med 2013; 79 - PI34
DOI: 10.1055/s-0033-1352124

Copaifera langsdorffii aqueous extract and its galloylquinic acid display gastroprotective activity

JC Da Costa 1, ED Motta 1, M Lemos 1, JK Bastos 1
  • 1University of São Paulo, Faculty of Pharmaceutical Science of Ribeirão Preto, São Paulo, Brazil.

Ulcer is a result of the imbalance between aggressive and protective factors in the stomach tissue. Copaifera langsdorffii Desf. is a tree that grows in Brazil and its oleo-resin has great potential for the treatment of gastric disorders based on the traditional use1. The aim of this study was to investigate the antiulcer activities of C. langsdorffii aqueous extract (AE) and its galloylquinic acid (GQ) compound. For that, the hydroalcoholic extract was partitioned with organic solvents and the remaining AE was lyophilized. AE was then submitted to Sephadex LH-20 using a gradient of methanol:water (1:9 to 9:1). A GQ was isolated from the Sephadex fractions by reverse phase high performance liquid chromatography (HPLC-RP). The compound was identified by nuclear magnetic resonance (NMR) means. The gastroprotective activity was evaluated using Ethanol/HCl induced ulcer model2. Balb-C male mice (n = 6, 20 ± 3 g) were orally treated with 30, 100 and 300 mg/kg of AE, and 30 mg/kg of GQ. Pantoprazole (30 mg/kg) and saline solution (0.9%) were used as controls. One hour after the treatment, animals received Ethanol/HCl (60%/0.03N) to induce stomach lesions, and one hour later animals were euthanized and lesions were analyzed. In the total extract, the AE corresponded to 28.7%. The GQ was isolated after collecting 411 fractions (20 mL) from Sephadex column, which was further purificated by HPLC-RP. The AE exhibited gastroprotective activities of 79%, 84% and 88%, at doses of 30, 100 and 300 mg/kg, respectively. Pantoprazole and GQ displayed 93.1% and 93.8% of gastroprotective activities, respectively. Therefore, the rich galloylquinic AE and its isolated GQ displayed remarkable gastroprotective effects. However, further studies are needed to confirm the mechanisms involved in the gastroprotection.

Acknowledgment: FAPESP

References:

[1] PAIVA, et al. J Ethnopharmacol 62 (1998) 73 – 78.

[2] MIZUI & DOTEUCHI Jpn J Pharmacol 33, (1983) 939 – 945.