Chemical constituents from the fermented broths of Coprinellus radians

In our preliminary screening, the ethyl acetate extracts of the fermented broths of Coprinellus radians ^#YMJ1168 were found to exhibit moderate growth inhibitory activity against A549 lung cancer cell line with a GI₅₀ value of 55.1 µg/mL. Thus, a broths of this fungus were carried out, which resulted in the isolation and identification of five compounds 1-5. Their structures were elucidated to be coprinol (1), guanacastanes P (2), J (3), E (4) and N (5) based on spectroscopic analyses. Among these, 1 and 2 were new, and 3 was isolated from natural resources for the first time. The growth inhibitory activities of 1, 3, 4, and 5 against A549 were evaluated, and only 2 exhibited moderate activity with a GI₅₀ value of 18.2µM. Moreover, by adding suberoylanilide hydroxamic acid (SAHA, 10µM), the histone deacetylase inhibitor, in the culture medium of this fungal strain, an unexpected component was found, which showed a molecular ion [M – H]^- at m/z 507 by LC/Q-TOFMS analysis. However, the structure of this component remained to be further investigated.