Five new components from the aerial part of Lindera akoensis with anti-inflammatory activity

Lindera akoensis (Lauraceae) is an endemic evergreen tree that grows in broad-leaved forests in lowlands throughout Taiwan. Aporphines, alkaloids, sesquiterpenoids, butanolides, furanoids, chalconoids, and phenolic compounds were widely distributed in the plants of the genus Lindera. Some isolates exhibit biological activies, including anti-mycobacterial, anti-inflammatory, anti-human lung cancer (SBC-3), osteoclast differentiation inhibitory, human slowing down of the pregession of diabetic nephropathy in mice, anti-nociceptive, and LDL anti-oxidation effect et al.. In this study, we have isolated five new compounds, 3β-((E)-dodec-1-enyl)-4β-hydroxy-5β-methyldihydrofuran-2-one (1), 3α-((E)-dodec-1-enyl)-4β-hydroxy-5β-methyldihydrofuran-2-one (2), linderinol (3), 4'-O-methylkaempferol 3-O-α-L-(4''-E-p-coumaroyl)rhamoside (4), kaempferol 3-O-α-L-(4''-Z-p-coumaroyl)rhamoside (5) as well as six known compounds. These components show in vitro anti-inflammatory activity decrease the LPS-stimulated product of nitrite in RAW 264.7 cell with IC₅₀ values of 1.4 – 67.9µM. (Indomethacin was used as positive control with IC₅₀ value of 182.9 µm).