Effect of Acute Stresses on Zebra Fish (Danio rerio) Metabolome Measured by NMR-Based Metabolomics

Mian Yahya Mushtaq; Rosilene Moretti Marçal; Danielle L. Champagne; Frank van der Kooy; Robert Verpoorte; Young Hae Choi

doi:10.1055/s-0034-1382878

Planta Medica, Table of Contents

Planta Med 2014; 80(14): 1227-1233
DOI: 10.1055/s-0034-1382878

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Effect of Acute Stresses on Zebra Fish (Danio rerio) Metabolome Measured by NMR-Based Metabolomics

Authors

Mian Yahya Mushtaq

¹Natural Products Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands
Rosilene Moretti Marçal

²Laboratorio de Farmacodinâmica e Etnofarmacologia (LAFETH), DEA, Universidade Federal de Sergipe-UFS, São Cristóvão, Brazil
Danielle L. Champagne

³Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands
Frank van der Kooy

¹Natural Products Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands
Robert Verpoorte

¹Natural Products Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands
Young Hae Choi

¹Natural Products Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands

Abstract

We applied an acute stress model to zebra fish in order to measure the changes in the metabolome due to biological stress. This was done by submitting the fish to fifteen minutes of acute confinement (netting) stress, and then five minutes for the open field and light/dark field tests. A polar extract of the zebra fish was then subjected to ¹H nuclear magnetic spectroscopy. Multivariate data analysis of the spectra showed a clear separation associated to a wide range of metabolites between zebra fish that were submitted to open field and light/dark field tests. Alanine, taurine, adenosine, creatine, lactate, and histidine were high in zebra fish to which the light/dark field test was applied, regardless of stress, while acetate and isoleucine/lipids appeared to be higher in zebra fish exposed to the open field test. These results show that any change in the environment, even for a small period of time, has a noticeable physiological impact. This research provides an insight of how different mechanisms are activated under different environments to maintain the homeostasis of the body. It should also contribute to establish zebra fish as a model for metabolomics studies.

Key words

zebra fish - NMR - metabolomics - stress

Full Text

References

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Supplementary Material

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