Acute Fasting-Induced Repression of the Hypothalamic-Pituitary-Gonadal Axis is Reversed by RF-9 Administration in the Adult Male Macaque

A. Batool; R. Naz; M. Wazir; A. Azam; R. Ullah; F. Wahab; M. Shahab

doi:10.1055/s-0034-1387788

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2014; 46(13): 927-832
DOI: 10.1055/s-0034-1387788

Endocrine Research

Acute Fasting-Induced Repression of the Hypothalamic-Pituitary-Gonadal Axis is Reversed by RF-9 Administration in the Adult Male Macaque

A. Batool

¹Laboratory of Reproductive Neuroendocrinology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

,

R. Naz

¹Laboratory of Reproductive Neuroendocrinology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

,

M. Wazir

¹Laboratory of Reproductive Neuroendocrinology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

,

A. Azam

¹Laboratory of Reproductive Neuroendocrinology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

,

R. Ullah

¹Laboratory of Reproductive Neuroendocrinology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

,

F. Wahab

²Department of Physiology, Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan

,

M. Shahab

¹Laboratory of Reproductive Neuroendocrinology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

› Institutsangaben

Abstract

Recently, hypothalamic RFRP-3 (a mammalian ortholog of avian GnIH) signaling has been proposed as an important negative modulator of the reproductive axis. The current study examined whether repression of reproductive hormonal expression during short-term fasting conditions in higher-order primate is influenced by altered RFRP-3 signaling. Eight intact postpubertal male macaques (Macaca mulatta) were administered a single intravenous bolus of RF-9 (n=4), a potent and putative RFRP-3 receptor antagonist, or vehicle (n=4) following a 48-h fasting condition. Intermittent blood samples were collected every 30 min during the 4-h post-bolus period, and blood glucose, plasma cortisol, and testosterone concentrations were measured. Relative to fed conditions, fasting reduced glucose and testosterone levels (p<0.005) and increased cortisol levels (p<0.05). Relative to baseline, mean testosterone levels were elevated 150 min after RF-9 (p<0.05) but not vehicle administration. In addition, elevated mean plasma testosterone levels following RF-9 administration were equivalent to levels observed in normal fed monkeys. These results suggest an important role for RFRP-3 signaling in conveying metabolic state information to the reproductive axis in higher primates.

Key words

RFRP-3 - RF-9 - testosterone - GnIH - monkey - fasting

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Referenzen

References
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