Planta Med 2014; 80 - P1L3
DOI: 10.1055/s-0034-1394661

Anti-inflammatory and immunomodulatory properties of polyphenolic compounds from Clusiaceae/Calophyllaceae: A focus on coumarins

C Rouger 1, S Derbré 1, M Litaudon 2, K Awang 3, B Charreau 4, P Richomme 1
  • 1EA921 SONAS/SFR4207 QUASAV, Université d'Angers, 10 r André Boquel, 49100 Angers, France
  • 2ICSN, CNRS, 1 Av de la Terrasse, 91198 Gif-sur-Yvette Cedex, France
  • 3University of Malaya, Department of Chemistry, Faculty of Science, 50603 Kuala Lumpur, Malaysia
  • 4INSERM, UMR1064, 30 Bd Jean Monnet, 44093 Nantes Cedex, France

Vascular endothelium plays a central role in the development of inflammatory and immune processes, which are involved in graft rejection [1]. Many Clusiaceae/Calophyllaceae species (pantropical plants) biosynthesize original polyphenolic compounds exhibiting antioxidant and anti-inflammatory properties [2 – 3]. Therefore, different Clusiaceae/Calophyllaceae polyphenols were selected in order to evaluate in vitro their anti-inflammatory and immunomodulatory potential towards Human Umbilical Vein Endothelial Cells (HUVECs). The VCAM-1, ICAM-1, E-selectin, HLA-I, HLA-II, HLA-E and MICA surface-expressions of HUVECs were evaluated by flow cytometry. It appeared that a few coumarins and one benzophenone at 10µM significantly inhibited the expression of several markers previously induced by TNF-α or IFN-γ cytokines, being more active that the immunosuppressive reference compound – zoledronic acid – at the same concentration. Among these active compounds, one phenylcoumarin isolated from the Malaysian Mesua lepidota T. Anderson (Calophyllaceae) was identified as a new one. By comparing biological effects with substitution patterns, preliminary structure-activity relationship was also established.

Keywords: Clusiaceae, Calophyllaceae, coumarins, inflammation, immunomodulation


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