Synergistic Effect of Fragrant Herbs in Japanese Scent Sachets

Abstract

The sedative activity of eight aromatic natural medicines that are traditionally used in Japanese scent sachets was examined using an open field test with mice. Galangal (Kaempferia galanga), patchouli (Pogostemon cablin), sandalwood (Santalum album), spikenard (Nardostachys chinensis), cinnamon (Cinnamomum cassia), clove (Syzygium aromaticum), star anise (Illicium verum), and borneol (Dryobalanops aromatica) distilled oils were used. These natural medicines have various pharmacological effects. For example, galangal has insecticidal activity and clove extracts possess strong total antioxidant activity. Aromatherapy, a well-known complementary medicine system that uses inhalation, has recently attracted much attention. The sedative activity of inhaled aromatic compounds or essential oils has been examined by measuring the spontaneous motor activity of mice in an open field test. The galangal, patchouli, sandalwood, spikenard, and borneol oils showed significant sedative effects. The effect was stronger for a mixture of the five oils than for any of the single oils. This suggests that the oil mixture may have synergistic activity. Sedative activity was not observed when inactive oils (cinnamon, clove, and star anise) were added to the mixture of the five active oils.

Key words

sedative effect - inhalation - fragrant herbs - synergistic effect

• References

• 1 Pandji C, Grimm C, Wray V, Witte L, Proksch P. Insecticidal constituents from four species of the Zingiberaceae. Phytochemistry 1993; 34: 415-419
• 2 Gulcin I, Sat IG, Beydemir S, Elmastas M, Kufrevioglu OI. Comparison of antioxidant activity of clove (Eugenia caryophylata Thunb) buds and lavender (Lavandula stoechas L.). Food Chem 2004; 87: 393-400
• 3 Cavanagh HMA, Wilkinson JM. Biological activities of lavender essential oil. Phytother Res 2002; 16: 301-308
• 4 Ebihara T, Ebihara S, Maruyama M, Kobayashi M, Itou A, Arai H, Sasaki H. A randomized trial of olfactory stimulation using black pepper oil in older people with swallowing dysfunction. J Am Geriatr Soc 2006; 54: 1401-1406
• 5 Buchbauer G, Jirovetz L, Jager W, Plank C, Dietrich H. Fragrance compounds and essential oils with sedative effects upon inhalation. J Pharm Sci 1993; 82: 660-664
• 6 Takemoto H, Ito M, Shiraki T, Yagura T, Honda G. Sedative effects of vapor inhalation of agarwood oil and spikenard extract and identification of their active components. J Nat Med 2008; 62: 41-46
• 7 Ohmori A, Shinomiya K, Utsu Y, Tokunaga S, Hasegawa Y, Kamei C. Effect of santalol on the sleep-wake cycle in sleep-disturbed rats. Nihon Shinkei Seishin Yakurigaku Zasshi 2007; 27: 167-171
• 8 Takemoto H, Yagura T, Ito M. Evaluation of volatile components from spikenard: valerena-4, 7(11)-diene is a highly active sedative compound. J Nat Med 2009; 63: 380-385
• 9 Honda G, Koezuka Y, Kamisako W, Tabata M. Isolation of sedative principles from Perilla frutescens . Chem Pharm Bull 1986; 34: 1672-1677
• 10 Ito N, Nagai T, Oikawa T, Yamada H, Hanawa T. Antidepressant-like effect of l-perillaldehyde in stress-induced depression-like model mice through regulation of the olfactory nervous system. Evid Based Complement Alternat Med 2011; 2011: 512697
• 11 Huang L, Yagura T, Chen S. Sedative activity of hexane extract of Keampferia galanga L. and its active compounds. Ethnopharmacol 2008; 120: 123-125
• 12 Ito K, Ito M. Sedative effects of vapor inhalation of the essential oil of Microtoena patchoulii and its related compounds. J Nat Med 2011; 65: 336-343
• 13 Misra BB, Dey S. Evaluation of in vivo anti-hyperglycemic and antioxidant potentials of α-santalol and sandalwood oil. Phytomedicine 2013; 20: 409-416
• 14 Misra BB, Dey S. Biological activities of East Indian sandalwood tree, Santalum album . Peer J PrePrints 2013; 1: e96v1
• 15 Tianpeng Y, Min T, Qiaoqiong L, Guangwu L. Mechanisms of eugenol improving learning and memory through olfactory pathway on Kunming mice. Zhongguo Kangfu Yixue Zazhi 2007; 22: 487-489
• 16 Kagawa D, Jokura H, Ochiai R, Tokimitsu I, Tsubone H. The sedative effects and mechanism of action of cedrol inhalation with behavioral pharmacological evaluation. Planta Med 2003; 69: 637-641
• 17 Serizawa S, Miyamichi K, Nakatani H, Suzuki M, Saito M, Yoshihara Y, Sakano H. Negative feedback regulation ensures the one receptor–one olfactory neuron rule in mouse. Science 2003; 302: 2088-2094
• 18 Iwasaki Y, Tanabe M, Kobata K, Watanabe T. TRPA1 agonists-allyl isothiocyanate and cinnamaldehyde-induce adrenaline secretion. Biotechnol Biochem 2008; 72: 2608-2614
• 19 Jones DL, Mogenson GJ, Wu M. Injections of dopaminergic, cholinergic, serotoninergic and gabaergic drugs into the nucleus accumbens: effects on locomotor activity in the rat. Neuropharmacology 1981; 20: 29-37
• 20 Burden RL, Faires JD. Numerical analysis, 9th edition. Boston: Brooks/Cole; 2010: 193-202