Mycosporine-Like Compounds in Chlorolichens: Isolation from Dermatocarpon luridum and Dermatocarpon miniatum, and their Photoprotective Properties

Thi Thu Tram Nguyen; Marylène Chollet-Krugler; Françoise Lohézic-Le Dévéhat; Isabelle Rouaud; Joël Boustie

doi:10.1055/s-0034-1396321

Planta Medica Letters, Table of Contents

Planta Medica Letters 2015; 2(1): e1-e5
DOI: 10.1055/s-0034-1396321

Letter

Georg Thieme Verlag KG Stuttgart · New York

Mycosporine-Like Compounds in Chlorolichens: Isolation from Dermatocarpon luridum and Dermatocarpon miniatum, and their Photoprotective Properties

Authors

Thi Thu Tram Nguyen

Institut des Sciences Chimiques de Rennes, UMR CNRS 6226, Equipe PNSCM «Produits Naturels – Synthèses – Chimie Médicinale», UFR Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, Université Européenne de Bretagne, Rennes, France
Marylène Chollet-Krugler

Institut des Sciences Chimiques de Rennes, UMR CNRS 6226, Equipe PNSCM «Produits Naturels – Synthèses – Chimie Médicinale», UFR Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, Université Européenne de Bretagne, Rennes, France
Françoise Lohézic-Le Dévéhat

Institut des Sciences Chimiques de Rennes, UMR CNRS 6226, Equipe PNSCM «Produits Naturels – Synthèses – Chimie Médicinale», UFR Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, Université Européenne de Bretagne, Rennes, France
Isabelle Rouaud

Institut des Sciences Chimiques de Rennes, UMR CNRS 6226, Equipe PNSCM «Produits Naturels – Synthèses – Chimie Médicinale», UFR Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, Université Européenne de Bretagne, Rennes, France
Joël Boustie

Institut des Sciences Chimiques de Rennes, UMR CNRS 6226, Equipe PNSCM «Produits Naturels – Synthèses – Chimie Médicinale», UFR Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, Université Européenne de Bretagne, Rennes, France

Abstract

Two mycosporines were isolated for the first time in two chlorolichen species, Dermatocarpon luridum and Dermatocarpon miniatum. Mycosporine glutaminol (1) and mycosporine glutamicol (2) were isolated along with the ethyl ester of mycosporine glutamicol (3), which was formed during the purification process. Aqueous extracts and pure mycosporines were then investigated for their antioxidant activities and photoprotective properties along with their photostability and photocytotoxicity. Semi-purified mycosporine fractions were much more antioxidant than lichen aqueous crude extracts. Compound 3 (IC₅₀ = 4.00 µg/mL) was found to be as active as quercetin (IC₅₀ = 6.75 µg/mL), while the two genuine mycosporines 1 and 2 exhibited moderate activity. The three mycosporines were found to be stable until 5J/m² UVA and UVB radiations whereas Trolox, used as a positive control, was degraded up to 10 % and 19 %, respectively. Moreover, these mycosporines and semi-purified extracts did not induce any phototoxicity on HaCaT cells exposed to UVA radiations.

Key words

Dermatocarpon luridum - Dermatocarpon miniatum - Verrucariaceae - lichen - mycosporine-like amino acids (MAAs) - photoprotection

Full Text

References

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