The Role of Single Nucleotide Polymorphisms in C-type Lectin Receptors for Susceptibility towards Pulmonary Tuberculosis in Hyderabad (India)
C-type lectin receptors (CTLRs) are a type of Pattern Recognition Receptors (PRRs) expressed in various immune cells that have been newly found to play important role in recognition of Mycobacterium tuberculosis (Mtb). The CTRLs in the Dectin-1 & Dectin-2 clusters on human chromosome 12 are of particular interest. Recently it was found that Mincle & MCL detect mycobacterial cord factor & Dectin-2 directly recognizes the Mannose capped Lipoarabinomannan. Several PRRs have been studied for the role of their SNPs in individual susceptibility to TB. However, no genetic studies have been done in humans for SNPs in these important CTLRs that link them to TB. The aim of our study is to find the association between the relevant SNPs in various CTLRs and TB in a case-control study. We generated a cohort of 145 pulmonary TB cases registered under the Revised National TB Control Program in Hyderabad, India & 241 healthy controls. In a pilot study, Next Generation Sequencing was performed targeting the whole genes from Dectin-1 & Dectin-2 clusters and known SNPs in other CTLRs related to TB. After various statistical adjustments, we annotated about 60 SNPs (known and unknown) that could be associated with the susceptibility or resistance to TB in those cases. We plan to sequence all the samples for these SNPs. In case of positive correlation we will perform a functional analysis of the SNPs that were mostly associated with the risk of pulmonary Tb. Our results will add an important impact for elucidating the role of SNPs in CTLRs genes in patients with pulmonary tuberculosis.
*) contributed equally.