Topical Treatment of Hair Loss with Formononetin by Modulating Apoptosis

Mi Hye Kim; You Yeon Choi; Ji Eun Lee; Kyuseok Kim; Woong Mo Yang

doi:10.1055/s-0035-1557897

Planta Medica, Inhaltsverzeichnis

Planta Med 2016; 82(01/02): 65-69
DOI: 10.1055/s-0035-1557897

Biological and Pharmacological Activitiy

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Topical Treatment of Hair Loss with Formononetin by Modulating Apoptosis

Autoren

Mi Hye Kim

¹College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University, Seoul, Korea
You Yeon Choi

¹College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University, Seoul, Korea
Ji Eun Lee

¹College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University, Seoul, Korea
Kyuseok Kim

²Department of Ophthalmology, Otorhinolaryngology and Dermatology of Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea
Woong Mo Yang

¹College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University, Seoul, Korea

Abstract

Formononetin is one of the main components of red clover plants and its role on hair regrowth against hair loss has not been established yet. In the present study, we assessed the potential effects of formononetin on alopecia, along with impaired hair cycles by induction of apoptosis-regression.

Depilated C57BL/6 mice were used for monitoring the hair cycles. Formononetin (1 and 100 µM) was topically treated to the dorsal skin for 14 days. Topical formononetin treatment induced miniaturized hair follicles to recover to normal sizes. Tapering hair shaft began to grow newly, emerging from the hair follicles by formononetin. In addition, formononetin inhibited the activation of caspase-8 and decreased the procaspase-9 expression. As a result of formononetin treatment, anti-apoptotic Bcl-2 was up-regulated, whereas pro-apoptotic Bax and p53 were down-regulated, resulting in a decrease of caspase-3 activation. Formononetin showed the obvious inhibition of apoptosis under terminal deoxynucleotidyl transferase dUTP nick end labeling staining thereafter.

Taken together, our findings demonstrate that formononetin exerted the hair regrowth effect on hair loss, in which the underlying mechanisms were associated with Fas/Fas L-induced caspase activation, thus inhibiting apoptosis.

Key words

formononetin - hair loss - hair cycle - apoptosis - caspase

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Referenzen

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