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Planta Medica Letters 2015; 2(01): e42-e47
DOI: 10.1055/s-0035-1558100
Letter
Georg Thieme Verlag KG Stuttgart · New York

Interaction of Vietnamese Medicinal Plant Extracts with Recombinantly Expressed Human Neurokinin-1 Receptor

Long Doan Dinh
1   VNU School of Medicine and Pharmacy, Vietnam National University, Hanoi, Vietnam
2   Key Laboratory of Protein and Enzyme Technology, VNU University of Science, Hanoi, Vietnam
,
Nhung Hong Thi Pham
1   VNU School of Medicine and Pharmacy, Vietnam National University, Hanoi, Vietnam
2   Key Laboratory of Protein and Enzyme Technology, VNU University of Science, Hanoi, Vietnam
,
Nhung My Thi Hoang
2   Key Laboratory of Protein and Enzyme Technology, VNU University of Science, Hanoi, Vietnam
,
Cuong Trinh Tat
2   Key Laboratory of Protein and Enzyme Technology, VNU University of Science, Hanoi, Vietnam
,
Van Hong Thi Nguyen
2   Key Laboratory of Protein and Enzyme Technology, VNU University of Science, Hanoi, Vietnam
,
Lan Thuong Thi Vo
2   Key Laboratory of Protein and Enzyme Technology, VNU University of Science, Hanoi, Vietnam
,
Huyen Thanh Pham
3   Vietnam National Institute of Medicinal Materials, Hanoi, Vietnam
,
Kenneth Lundstrom
4   PanTherapeutics, Lutry, Switzerland
› Author Affiliations
Further Information

Publication History

received 19 May 2015
revised 20 August 2015

accepted 23 August 2015

Publication Date:
06 November 2015 (online)

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Abstract

A primary functional receptor screening assay was performed to examine the effects of methanolic extracts from ten Vietnamese medicinal plants on the human neurokinin-1 receptor expressed from Semliki Forest virus vectors in Chinese hamster ovary cells. Extracts from Piper nigrum, Stephania cambodica and Styphnolobium japonicum were found to exert inhibition on agonist-induced human neurokinin-1 receptor activity. Secondary assays and high performance liquid chromatography of the lead compounds addressed a possible association between pharmacological responses and these chemical compounds. Strong inhibition of human neurokinin-1 receptor was observed for extracts revealing the highest inhibitory potency for rotundine (tetrahydropalmatine) in S. cambodica extracts with IC50 of 0.88 µM, followed by piperine and capsaicin in P. nigrum extracts with IC50 values of approximately 2 µM, whereas rutin in S. japonicum extracts failed to inhibit hNK1R up to 100 µM.

Key words

Piper nigrum - Stephania cambodica - Styphnolobium japonicum - Piperaceae - Fabaceae - Menispermaceae - piperine - capsaicin - rotundine - rutin - neurokinin-1 receptor - functional assays
 

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