Thorac cardiovasc Surg 2018; 66(01): 083-090
DOI: 10.1055/s-0035-1564689
Original Basic Science
Georg Thieme Verlag KG Stuttgart · New York

Periodontal Bacterial DNA and Their Link to Human Cardiac Tissue: Findings of a Pilot Study

Dirk Ziebolz
Department of Cariology, Endodontology and Periodontology, University of Leipzig, Leipzig, Germany
,
Christoph Rost
Department of Preventive Dentistry, Periodontology and Cariology, University Medical Center of Göttingen, Göttingen, Germany
,
Julia Schmidt
Department of Preventive Dentistry, Periodontology and Cariology, University Medical Center of Göttingen, Göttingen, Germany
,
Regina Waldmann-Beushausen
Department of Thoracic and Cardiovascular Surgery, University Medical Center of Göttingen, Göttingen, Germany
,
Friedrich A. Schöndube
Department of Thoracic and Cardiovascular Surgery, University Medical Center of Göttingen, Göttingen, Germany
,
Rainer F. Mausberg
Department of Preventive Dentistry, Periodontology and Cariology, University Medical Center of Göttingen, Göttingen, Germany
,
Bernhard C. Danner
Department of Thoracic and Cardiovascular Surgery, University Medical Center of Göttingen, Göttingen, Germany
› Author Affiliations
Further Information

Publication History

22 May 2015

17 August 2015

Publication Date:
05 October 2015 (eFirst)

Abstract

Background The aim of this pilot study was to detect correlations of microbiological DNA, inflammatory proteins, and infection parameters in patients with periodontal disease (PD) and valvular heart disease (VHD).

Methods A perioperative comprehensive dental examination for the investigation of periodontal status, including sampling of specific subgingival bacteria, was performed in 10 patients with indication for surgery of aortic valve stenosis with or without concomitant myocardial revascularization. Standard protocol biopsies were taken from right atrium (A), left septal myocardium (M), and aortic valve (V). Eleven periodontal pathogens DNA in oral and cardiac tissue samples (A/M/V) were analyzed using polymerase chain reaction. For cardiac tissue samples, Western blot analysis of LPS-binding protein (LBP), immunohistochemical (IHC) detection of LBP-big42, LPS-binding protein receptor (CD14), and macrophages (CD68), as well as inflammation scoring measurement were performed.

Results Periodontitis was present in all patients with severe intensity in 7, moderate in 2 and mild in one patient. Same bacterial DNA was detected in A, M, and V in different distribution, and detection was more often in atrium than in myocardium or valve tissue. Morphological investigation revealed increased extracellular inflammatory cell migration. In IHC markers of LBP, CD68 and CD14 showed positive findings for all patients in atrium and myocardium.

Conclusion Our results demonstrate the presence of oral bacterial DNA in human cardiac tissue, as well as inflammatory markers potentially indicating connection of PD and VHD. Further investigation is necessary to confirm these preliminary data.

Funding

This research received no grant from any funding agency in the public, commercial, or not-for-profit sectors.