Planta Med 2016; 82 - OA21
DOI: 10.1055/s-0036-1578591

Experimental Design for the Characterization and Quantitation of Antrodia cinnamomea Triterpenoids: A Tough Case to Crack

TY Wu 1, 2, M El-Shazly 1, 3, FR Chang 1, YC Wu 1, 2, 4
  • 1Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
  • 2Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan
  • 3Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt
  • 4School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan

Antrodia cinnamomea (AC) is one of the Fareast treasured medicinal mushrooms [1]. Among different AC components, triterpenoids are considered the most therapeutically important constituents due their cytotoxic and anti-inflammatory activities. In the current investigation, we proposed a mathematical and statistical extraction protocol for evaluating the concentrations of the total ergostane and lanostane triterpenoid derivatives, from the ethanolic extract of the wild fruiting bodies of AC (EEAC) by using response surface methodology (RSM) and quantitative NMR (qNMR). The optimum response surface model illustrated that the variations of the studied response variables reached more than 90% indicating the accuracy of the developed model in explaining the variability of responses. On the other hand, the quantification of EEAC total triterpenoids was performed by comparing the HPLC-tandem MS results with those of the qNMR results. The accuracy of the used techniques was also evaluated. The experimental design of EEAC optimum extraction procedure obtained using RSM and qNMR allowed the accurate characterization and quantitation of AC triterpenoids.

Fig. 1

Acknowledgements: This work was supported by grants from the Department of Health, Executive Yuan, Taiwan (DOH100-TD-C-111 – 002) and the National Science Council, Taiwan awarded to Yang-Chang Wu and Fang-Rong Chang.

References: [1] Mei-Chin Lu, Yang-Chang Wu, et al. (2013) Pharmacol. & Therapeut, 139: 124 – 156.