Planta Med 2016; 82 - OA24
DOI: 10.1055/s-0036-1578594

Platelet Enhancing And Immunomodulatory Activity Of Carica Papaya Leaves

SH Ansari 1, V Anjum 1, S Ahmad 1
  • 1Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi – 110062, India

The aim is to examine the effect of standardised aqueous extract of leaves of Carica papaya (CPL) on platelet count, extra medullary haematopoiesis (EMH) and immunomodulation after oral administration. The extract was standardized by quantification of myrecitin, caffeic acid, trans ferulic acid, kaempferol using HPLTC followed by development of UPLC-QToF fingerprinting. The effects of CPL on proliferative response of platelet, total leukocyte count (TLC), differential leukocyte count (DLC), up to 14 days in wistar rat however immunomodulation for delayed type of hypersensitivity (DTH) [1], heamagglutination titre (HT), and in vivo carbon clearance [2] up to 10 days in albino mice against cyclophosphamide. The HPTLC analysis results in identification of myrecitin, caffeic acid, trans ferulic acid and kaempferol at Rf value 0.39 ± 0.01, 0.44 ± 0.02, 0.50 ± 0.01 and 0.55 ± 0.01, respectively followed by tentative identification of 24 compounds using UPLC-QToF. Significant increase in thrombocytes were observed after oral administration of CPL (150 mg/Kg) against cyclophosphamide induced thrombocytopenia. There was significant (p < 0.001) increase in the DTH response; however, HT showed modulatory effect at 150 mg/Kg along with increase in phagocytic index. CPL showed maximum EMH release [3] as compared to toxic and normal groups with minimal fibrosis thickening in spleen at subcapsular region followed by minimal effect on megakaryocytes and less dense cell in white pulp region of spleen as observed in histopathology. It can be concluded that CPL can mediate the release of platelet count from megakaryocytes and stimulate antithrombocytopenic effect providing the means for the treatment and prevention of Dengue.

Acknowledgements: This research work financially supported by AICTE and DST, Ministry of Science and Technology, Government of India, New Delhi.

References: [1] Bin-Hafeez B, Ahmad I et al. (2001). J Ethnopharmacol, 75, 8 – 13. [2] Gokhale AB, Damre AS et al. (2003)J Ethnopharmacol, 84, 109 – 114. [3] Brunicardi FC, Anderson DK et al. (2009) McGraw-Hill Professional.