Diabetologie und Stoffwechsel 2016; 11 - P225
DOI: 10.1055/s-0036-1580972

Effect of Biochanin A on serum nesfatin-1 Level in STZ induced type 1 diabetic rat

M Eskandari Mehrabadi 1, Z Salemi 2
  • 1Arak University of Medical Sciences, Arak, Iran, Islamic Republic of
  • 2Arak University of Medical Sciences, Medical Biochemistry, Arak, Iran, Islamic Republic of

Introductions: Hypoglycemic effect of bioflavonoids have been approved, they can act as insulin secretagogues or insulin mimetics. Nesfatin-1 is a recently discovered regulatory peptide and affects glucose metabolism by increasing insulin sensitivity. In this study we investigated effect of Biochanin A (an isoflavonoid) on serum nesfatin-1 level in streptozotocin (STZ)-induced type 1 diabetic rats.

Material and methods: 30 male Wistar rats were divided randomly into five groups. Two groups of them were selected randomly as control. Group1: normal control (receiving 0.5% DMSO), Group2: normal control that receiving 10 mg/kg bw. biochanin A and diabetes was induced in a single injection of STZ (55 mg/kg BW). Diabetic rats randomly divided as follow: Group3: diabetic control receiving 0.5% DMSO), Group 4 and 5 were treated respectively with 10 and 15 mg/kg bw biochanin A. FBG (fasting blood glucose), insulin, nesfatin-1 were measured in all groups after 6 weeks.

Results: FBG was reduced and serum insulin level was increased significantly in treated rats compared to the diabetic control. Serum nesfatin-1 level was significantly increased in diabetic rats treated with 15 mg/kg biochanin A compared to the diabetic control and normal control. Nesfatin -1 was increased in diabetic control compered to normal control, but not significant.

Conclusion: Biochanin A has potential hypoglycemic effect, probably by increasing insulin and nesfatin-1 level. Biochanin A at 15 mg/kg was more effective than 10 mg/kg in increasing nesfatin-1 level.