Sirtuin 1 Levels in Recurrent Implantation Failure

Yaprak Engin-Ustun; A. Seval Ozgu-Erdinc; Emel Kiyak Caglayan; Cavidan Gulerman; Esma Sarikaya; Ayla Aktulay; Canan Demirtas; Salim Erkaya; Nafiye Yilmaz

doi:10.1055/s-0037-1606349

Revista Brasileira de Ginecologia e Obstetrícia / RBGO Gynecology and Obstetrics, Inhaltsverzeichnis

CC-BY-NC-ND 4.0 · Rev Bras Ginecol Obstet 2017; 39(10): 541-544
DOI: 10.1055/s-0037-1606349

Original Article

Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil

Sirtuin 1 Levels in Recurrent Implantation Failure

Níveis de sirtuína 1 em falhas recorrentes de implantação

Authors

Yaprak Engin-Ustun

¹Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
A. Seval Ozgu-Erdinc

¹Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
Emel Kiyak Caglayan

¹Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
Cavidan Gulerman

¹Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
Esma Sarikaya

¹Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
Ayla Aktulay

¹Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
Canan Demirtas

²Biochemistry Department, Gazi University, Ankara, Turkey
Salim Erkaya

¹Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
Nafiye Yilmaz

¹Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey

Abstract

Sirtuin 1 has an important role in cellular processes, including apoptosis and cellular stress. The purpose of this study was to assess serum sirtuin 1 levels in women with recurrent implantation failure (RIF). In this cross-sectional study, we included 28 women with RIF, 29 healthy women who had conceived by in vitro fertilization (IVF), and 30 women with a 1-cycle failure of IVF as controls. Human serum nicotinamide adenine dinucleotide (NAD)-dependent deacetylase sirtuin-1 (SIRT1/SIRT2L1) levels were detected using a commercial colorimetric kit. Recurrent implantation failure patients have higher sirtuin 1 levels than non-pregnant women and healthy pregnant women, but this difference did not reach statistical significance due to the low number of patients in our study. These higher sirtuin 1 levels may result from the inflammation imbalance of RIF patients. The only statistically significant correlation found was between age and sirtuin (r = 0.277, p = 0.009).

Resumo

A sirtuína 1 tem importante função nos processos celulares, incluindo a apoptose e o estresse celular. O objetivo deste estudo é o de avaliar níveis de sirtuína 1 em mulheres com falhas recorrentes de implantação (FRI). Neste estudo cruzado, incluímos 28 mulheres com FRI, 29 mulheres saudáveis que deram à luz por fertilização in vitro (FIV) bem-sucedida, e 30 mulheres com 1 ciclo de FIV malsucedido como controle. Os níveis de sirtuína 1 em soro humano de desacetilase dependente de dinucleotídeo de nicotinamida adenina (DNA) (SIRT1/SIRT2L1) foram detectados usando um kit colorimétrico comercial. Pacientes com FRI tiveram níveis de sirtuína 1 superiores às pacientes grávidas e aos controles, mas esta diferença não atingiu significância estatística devido ao baixo número de pacientes envolvidos. Estes níveis mais altos de sirtuína 1 podem ser resultado da inflamação desigual em pacientes com FRI. A única correlação estatisticamente significante encontrada foi entre idade e sirtuína (r = 0,277, p = 0,009).

Keywords

recurrent implantation failure - sirtuin - pregnancy - in vitro fertilization

Palavras-chave

falhas recorrentes de inseminação - sirtuína - gravidez - fertilização in vitro

Volltext

Referenzen

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