Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608183
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Diversity of diterpenoids and polyphenols in Salvia glutinosa L. analyzed using targeted metabolomics based on LC-qTOF-MS and 1H-NMR

S Ślusarczyk
1   Dept. Biochemistry, IUNG-Institute of Plant Cultivation and Soil Science, Pulawy, Poland
2   Dept. Pharmaceutical Biology, Botanical Garden of Medicinal Plants, Wroclaw Medical University, Poland, Wroclaw, Poland
,
J JaŘpińska
2   Dept. Pharmaceutical Biology, Botanical Garden of Medicinal Plants, Wroclaw Medical University, Poland, Wroclaw, Poland
,
Ł Pecio
1   Dept. Biochemistry, IUNG-Institute of Plant Cultivation and Soil Science, Pulawy, Poland
,
A Stochmal
1   Dept. Biochemistry, IUNG-Institute of Plant Cultivation and Soil Science, Pulawy, Poland
,
E Gille
3   National Institute of Research and Development for Biological Sciences/“Stejarul” Biological Research Center, Piatra-Neamt, Romania
,
A Matkowski
2   Dept. Pharmaceutical Biology, Botanical Garden of Medicinal Plants, Wroclaw Medical University, Poland, Wroclaw, Poland
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Salvia glutinosa L. (Sticky sage) is a vigorous perennial associated with lower montane deciduous forests in Europe and its roots can be an alternative source of tanshinones. To date, it has largely been ignored as a medicinal plant. Also, despite a couple of reports about its phytochemistry based on single samples, the natural diversity of potentially active constituents in this plant is little documented. Here, we used two analytic approaches, 1HNMR and UHPLC-qTOF-MS to get insight into the major and subtle differences in diterpenoid and phenylpropanoid profiles. Plant samples from three populations were tested – two East Carpathian – (1) from Romanian Bucovina, (2) from Ukrainian Skolivs'ki Beskydy, and (3) from the ex situ collection in the Botanical Garden of WMU in Wroclaw, Poland – started from material obtained in a westernmost Carpathian habitat in Beskid-Slaski. Additionally, we performed microplot experiment to test the influence of salt stress on the plant growth and phytochemical profile. Analytic data were processed using chemometric methods.

Samples of different origins were well separated by OPLS discriminant analysis both using NMR and LC-MS profiling. However, in NMR, the signals responsible for the differences were not assignable to any of specific metabolites. In LC-MS, the main differences were quantitative, such as in the content of salviaflaside or melitric acid B, and to lesser extent to tanshinone-like nor-abietanoids. The salt stress induced by 50 and 100 mmol NaCl caused growth retardation and lower biomass (between 10 and 15%) without any significant impact on specialized metabolites composition. The addition of silica alleviated negative impact of excess salt in the soil.

Salvia glutinosa root is a source of pharmacologically relevant tanshinones and phynylpropanoid. The samples from various Carpathian in situ and ex situ populations are not significantly different from the viewpoint of phytochemical value even if they can be easily discriminated using multivariate statistics.

Financial support: Polish National Science Center FUGA – DEC/2014/12/S/NZ9/00715.