Kampferol-3 – 0 -glucopyranoside identified as the major Anti-oxidant glycoside from the Red kidney Bean (Phaseolus vulgaris) seed commonly consumed in Eastern Nigeria.

Plants and food consumed by man represents sources for human disease management. Aqueous and aqueous Methanolic extracts (80% Methanol) of the Nigeria grown red kidney beans, both showed the presence of one major compound. In this study, Kampferol-3 – 0 -glucopyranoside was isolated as the major and active compound. The structure was determined based on UV, MS, 1 H-NMR and 13C-NMR spectroscopic data. The HPLC chromatogram of both extracts and compounds reveals a single identical significantly major peak which is the major glycoside. In the bioassay with DPPH and superoxide scavenging activity, both water and aqueous methanolic extract with the purified compound showed significant activity; having IC-50 of 310, 187 and 10 µg/ml respectively; as against 25 µg/ml of standard antioxidant used. Oxygen free radicals, the reactive oxygen species (ROS) are also involved in cancer, cardiovascular diseases and various chronic liver diseases. Potent antioxidants quench these ROS aggravated degeneration. Our results revealed an insight for exploration of Kampferol-3 – 0 -glucopyranoside as an active compound for future anti-cancer therapeutics.