Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608463
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

The antioxidative intervention in colon cancer initiation by Detarium microcarpum and its fractions

A Akinsanmi
1   African Center of Excellence in Phytomedicine Research and Development, Faculty of Pharmaceutical Sciences, University of Jos, Nigeria, Jos, Nigeria
,
T Johnson
2   University of Jos, Faculty of Medical Sciences, Department of Biochemistry, Jos, Nigeria
,
J Aguiyi
1   African Center of Excellence in Phytomedicine Research and Development, Faculty of Pharmaceutical Sciences, University of Jos, Nigeria, Jos, Nigeria
3   University of Jos, Faculty of Pharmaceutical Sciences,Department of Pharmacology, Jos, Nigeria
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Pathophysiological situations have shown that oxidative stress and inflammation are closely related. Evidences to show the simultaneous existence of chronic inflammation and oxidative stress in cardiovascular, neurodegenerative, diabetic, and chronic kidney diseases, do exist [1]. Whenever chronic inflammation takes place, pro-inflammatory molecules, are all upregulated. Thus, providing an enabling enviroment, for the exponential growth of malignant cells [2]. The uses of anti-inflammatory agents have been found to reduce the incidence and management of cancer. Three folkloric plants used in the treatment of inflammation and cancer were studied; Boswellia dalzielii, Detarium microcarpum, and Daniellia oliveri. Identification, authentication and voucher numbers were carried out at the Federal College of Forestry, Jos, Nigeria. For the selection phase, after the preliminary screening and antioxidative assays, DML had the highest inhibitory ability to prevent the formation of malondialdehyde in the liver, brain and colon; going by its low IC50. The in vitro antioxidative capacity was carried out among the Methanolic extract DML, Butanol fraction of DML (BfDML) and Ethyl acetate fraction of DML (EfDML).The result showed that EfDML had the highest antioxidative capacity, followed by DML and the least was BFDML. The EfDML was chosen and the acute toxicity was carried out as to determine the LD50. The test was carried out following the method described by the Organisation for Economic Cooperation and Development, OECD [3]. From the clinical observations, there was no sign of morbidity, but the results from the haematological, serum Biochemistry and histopathological studies, indicated the LD50 for EfDML extract to be ≤1000 mg/kg.

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[3] OECD (2001) OECD guideline for the testing of Chemicals, Acute Oral Toxicity- Acute Toxic Class Method [Guideline 423 adopted 17th December 2001].