Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608499
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

DETERMINATION OF PHENOLIC COMPOUNDS IN Calendula officinalis L. FLOWER'S

C Marques Fornseca Maira
1   Empresa de Pesquisa Agropecuária de Minas Gerais, Viçosa, Brazil
,
R Gonçalves Rodrigues Das Dores
2   Universidade Federal de Ouro Preto, OURO PRETO, Brazil
,
C Silva e Souza
3   Universidade Federal de Viçosa, Viçosa, Brazil
,
A Nogueira Sedyama Maria
1   Empresa de Pesquisa Agropecuária de Minas Gerais, Viçosa, Brazil
,
Y Poltronieri
1   Empresa de Pesquisa Agropecuária de Minas Gerais, Viçosa, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Calendula is cultivated in Brazil and widely used for medicinal purposes due to its healing, anti-inflammatory and antiseptic properties. Brazilian market does not provide vegetal drugs with biomarkers, within recommended range, thus, it is essential to study suitable technologies to produce high quantity and quality of vegetable raw. This work aimed to determinate the phenolic compound content in orange and yellow marigold flowers cultivated with increasing doses of cattle manure. The experiment was conducted at EPAMIG, Minas Gerais, Brazil. Marigold was cultivated in organic system using five doses of cattle manure (0; 30; 60; 90; 120tha-1). Flowers were harvested, dried in air circulating oven (40 °C) and macerated in ethanol. The ethanol was evaporated (40 °C) to obtain the crude extracts. The phenolic compounds were determinate by spectrophotometry (760nm). Crude extracts (5 mg) were diluted in ethanol PA (1000µL), where, 200µL were added to Folin-Ciocalteu reagent (1000µL) and 7.5% Na2CO3 (1600µL). The gallic acid was used in the standard curve and the results were expressed in mg/g. The mean contents of phenolic compounds were 679.20 ± 173.65 mg/g (CV = 9.67%). Higher concentrations of phenolic compounds (931.87 mg/g) were found in orange flowers without fertilization (0tha-1) and lower (368.53 mg/g) in yellow flowers fertilized with the highest dose of manure (120tha-1). In orange and yellow flowers extracts, treatments with fertilization of 30 t.ha-1 and without fertilization had higher levels of phenolic compounds. Possibly, these compounds were produced in response to stress and adaptive need to the cultivation conditions. The dose-response curve was inversely proportional to manure doses in all treatments. Yellow flowers and high doses of manure (90 and 120tha-1) do not increase the production of phenolic compounds.