Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608507
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Development of a colloidal gold nanoparticle based immunochromtographic strip for one step detection of puerarin and miroestrol

S Krittanai
1   Faculty of Pharmaceutical Science, Khon Kaen University, Khon Kaen, Thailand
2   Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand
,
T Kitisripanya
1   Faculty of Pharmaceutical Science, Khon Kaen University, Khon Kaen, Thailand
2   Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand
,
O Udomsin
1   Faculty of Pharmaceutical Science, Khon Kaen University, Khon Kaen, Thailand
2   Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand
,
W Putalun
1   Faculty of Pharmaceutical Science, Khon Kaen University, Khon Kaen, Thailand
2   Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Pueraria candollei Wall. ex Benth (White Kwao Krua) is a Thai traditional plant that presents phytoestrogenic effect. Puerarin and miroestrol are bioactive compounds in this plant. Puerarin can also found in other plant in genus Pueraria including Pueraria lobata (Kudzu). However, miroestrol was found only in P. candollei. Therefore, this compound can be used as identical marker of P. candollei. Nowadays, various products derived from these plants were existed in the market especially in Asian country. Some counterfeit product can be problematic for the health of product consumer. The effective method for determination chemical marker of the plant should be applied to ensure the product quality. This study aims to investigate the amount of puerarin and miroestrol in plant samples and derived products of P. candollei and P. lobata using two immunoassay based methods. An immunochromatographic strip colloidal gold based detection system was developed in this study. On a strip membrane, anti-puerarin polyclonal antibody and anti-miroestrol monoclonal antibody [1] were equipped in the test spots. Therefore, both compounds can be detected in one step. In addition, indirect competitive enzyme-linked immunosorbent assay (icELISA) was performed to confirm reliability of the developed strip. The result showed high agreement between two methods. Among the plant samples, level of puerarin and miroestrol contents were fluctuated according to plant species, cultivated location, and the part of plant. In conclusion, the developed strip is an interesting alternative method for testing puerarin and miroestrol in samples. The strip is compact so it facilitate analysis in work field. Analysis procedure is straight forward. It is not required any complex sample preparation and result can be obtained within 10 minutes after dipped the strip into sample solution.

Kitisripanya, T, Krittanai, S, Udomsin, O, Jutathis, K, Komaikul, J, Juengwatanatrakul, T, Putalun, W. Planta Med 2017; http://doi.org/10.1055/s-0043 – 102689.