Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608529
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Storage stability assessment of royal jelly skin care products by trans-10-hydroxy-2-decenoic acid (10-HDA) determination

A Uzunovic
1   The Control Laboratory of Agency for Medicinal Products and Medical Devices of Bosnia and Herzegovina, Sarajevo, Bosnia and Herzegovina
,
D Hatibovic
2   Faculty of Pharmacy, Travnik, Bosnia and Herzegovina
,
S Pilipovic
1   The Control Laboratory of Agency for Medicinal Products and Medical Devices of Bosnia and Herzegovina, Sarajevo, Bosnia and Herzegovina
,
A Sapcanin
3   Faculty of Pharmacy, Sarajevo, Bosnia and Herzegovina
,
I Tahirovic
4   Faculty of Science, Sarajevo, Bosnia and Herzegovina
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Royal jelly is an important bioactive compound that possesses several health promoting properties. It has been widely used in commercial medical products, healthy foods and cosmetics in many countries. Trans-10-hydroxy-2-decenoic acid (10-HDA) is the main fatty acid present in royal jelly and the 10-HDA content has been proposed as a freshness parameter for royal jelly quality analysis [1]. This work has been aimed to assess the 10-HDA stability (storage temperature 2 – 8 °C) in 5 different home-made royal jelly skin care products, presented in Table 1.

Tab. 1: Composition of royal jelly skin care products

Component

Sample 1

Sample 2

Sample 3

Sample 4

Sample 5

Frash royal jelly

5%w/w

5%w/w

10%w/w

Lyophilized royal jelly

5%w/w

5%w/w

Cream base

X

Sweet almond oil

X

X

X

X

Cocoa butter

X

X

X

X

Beeswax

X

X

X

X

Distilled water

X

X

X

X

X

Grape seed oil

X

X

X

X

X

Lavender oil

X

X

X

X

X

Bee pollen

2%w/w

Propolis

2%w/w

Honey

2%w/w

The HPLC method proposed by Kim et al. (2010) has been optimized and validated for the determination of 10-HDA in royal jelly skin care products [2]. All analyses were performed on a Waters Acquity UPLC system, equipped with a variable wavelength UV absorbance detector, operated with Waters EmpowerTM 2 software. UPLC analysis was performed using Agilent Poroshell 120 2,7um (100 mm x 4,6 mm) column, adjusted to 25 °C as a column temperature. The maximum absorbance of 10-HDA was confirmed to be 215nm. The mobile phase was a mixture of methanol, water, and phosphoric acid (55:45:2.7, v/v/v) and the flow rate was 1.0 mL/min. The total run time of each injected sample was 5 min, as indicated in the figure below. 10-HDA content in home-made royal jelly skin care products after 80 days varies from 0.52 ± 0.44 to 91.61 ± 1.73 percent of the initial content of 10-HDA (2 mg/g).

According to the results obtained in this study, we confirm the impact of change in composition on stability of the finished products.