Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608594
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Vernonia condensata: an active medicinal plant against Staphylococcus aureus strains

J Silva
1   Bioactive Natural Products Laboratory, Department of Biochemistry, Federal University of Juiz deFora, Rua José Lourenço Kelmer s/n, Campus Universitário, Bairro São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
M Bessa
1   Bioactive Natural Products Laboratory, Department of Biochemistry, Federal University of Juiz deFora, Rua José Lourenço Kelmer s/n, Campus Universitário, Bairro São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
V Tomasco
1   Bioactive Natural Products Laboratory, Department of Biochemistry, Federal University of Juiz deFora, Rua José Lourenço Kelmer s/n, Campus Universitário, Bairro São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
E Scio
1   Bioactive Natural Products Laboratory, Department of Biochemistry, Federal University of Juiz deFora, Rua José Lourenço Kelmer s/n, Campus Universitário, Bairro São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
MS Alves
2   Cellular and Molecular Bioactivity Laboratory, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Rua José Lourenço Kelmer s/n, Campus Universitário, Bairro São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
17 November 2017 (online)

 

Vernonia condensata Baker (Asteraceae Bercht. & Presl) is traditionally used to relief of several diseases, including those related to infectious disorders and caused by Staphylococcus aureus strains1 – 4. So, this study focused on the investigation of its antistaphylococcal activity. The leaves were extracted with ethanol and then submitted to a liquid-liquid partitioning process with solvents in increasing order of polarity. For this study, the ethyl acetate partition (PA) was used. The antibacterial activity was verified by the Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC)5 against five S. aureus ATCC® strains (6538, 25923, 29213, 33591, and 33592) and five methicillin-resistant S. aureus (MRSA)

clinical strains isolated from blood infections (1485279, 1605677, 1664534, 1688441, and 1830466). PA revealed antibacterial activity against all S. aureus strains tested, being more active against the ATCC® strains, with MIC value of 625 µg/mL. Considering the MRSA clinical strains, the MIC values were 1250 µg/mL (MRSA 1485279), 2500 µg/mL (MRSA 1688441 and MRSA 1830466), and > 5000 µg/mL (MRSA 1605677 and 1664534). Ampicillin and chloramphenicol were used as standard antibiotics. MBC was not found within the gradient concentration tested (5000 µg/mL to 7.8 µg/mL). Additionally, PA was considered bacteriostatic for all strains. In conclusion, it was possible to corroborate the potential of V. condensata as an antistaphylococcal agent, with bacteriostatic effect.

This work was supported by the grants from FAPEMIG, CAPES and CNPq.

[1] Frutuoso, V.S. et al Planta Medica, 60, 21 – 25, 1994.

[2] Risso, W. E. et al. Indian Journal of Experimental Biology, 48, 811 – 816, 2010.

[3] Silva, J. B. et al. Journal of Molecular Sciences, 12, 8993 – 9008, 2011.

[4] Valverde, A. L. Phytotherapy Research, 15, 263 – 264, 2001.

[5] Clinical and Laboratory Standards Institute. CLSI Document M100-S21, 2012.