Haemostatic Factors in Human Peripheral Afferent Lymph

George J. Miller; David J. Howarth; Janet C. Attfield; C. Justin Cooke; M. Nazeem Nanjee; Waldemar L. Olszewski; James H. Morrissey; Norman E. Miller

doi:10.1055/s-0037-1613832

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2000; 83(03): 427-432
DOI: 10.1055/s-0037-1613832

Review Article

Schattauer GmbH

Haemostatic Factors in Human Peripheral Afferent Lymph

George J. Miller

¹From the Medical Research Council Epidemiology and Medical Care Unit, London, UK

,

David J. Howarth

¹From the Medical Research Council Epidemiology and Medical Care Unit, London, UK

,

Janet C. Attfield

¹From the Medical Research Council Epidemiology and Medical Care Unit, London, UK

,

C. Justin Cooke

²Department of Cardiovascular Biochemistry, St Bartholomew ‘s and the Royal London School of Medicine and Dentistry, London, UK

,

M. Nazeem Nanjee

²Department of Cardiovascular Biochemistry, St Bartholomew ‘s and the Royal London School of Medicine and Dentistry, London, UK

,

Waldemar L. Olszewski

³Department of Surgical Research and Transplantology, Medical Research Council, Polish Academy of Sciences, Warsaw , Poland

,

James H. Morrissey

⁴Oklahom a Medical Research Foundation, Oklahoma City, Oklahoma, USA

,

Norman E. Miller

²Department of Cardiovascular Biochemistry, St Bartholomew ‘s and the Royal London School of Medicine and Dentistry, London, UK

› Author Affiliations

Abstract

Summary

Peripheral afferent lymph was obtained by cannulation of a collecting vessel in 17 healthy men (mean age 26 years). Lymph/plasma ratios of all vitamin K-dependent factors were lower than expected from molecular weight. Factor VII, factor IX and tissue factor pathway inhibitor (TFPI) lymph/plasma activity ratios were higher than antigen ratios. Activated factor VII (FVIIa) and TFPI-Xa complex concentrations were higher in lymph than plasma, and the raised FVIIa did not appear to be due to cannulation. The fibrinogen lymph/plasma activity (Clauss) ratio averaged about 20% of the antigen ratio. The result of an ELISA for D-dimer was higher in lymph than plasma, often more than five-fold. This high level in lymph was not explored but may indicate proteolysis of fibrinogen and fibrin with release of D-like and D-dimerlike fragments in interstitial fluid.

Keywords

Peripheral lymph - haemostatic factors - D-dimer

Full Text

References

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