Systemic Endothelial Cell Markers in Primary Antiphospholipid Syndrome

Frances M. K. Williams; Kiran Parmar; Graham R. V. Hughes; Beverley J. Hunt

doi:10.1055/s-0037-1614108

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2000; 84(05): 742-746
DOI: 10.1055/s-0037-1614108

Rapid Communication

Schattauer GmbH

Systemic Endothelial Cell Markers in Primary Antiphospholipid Syndrome

Authors

Frances M. K. Williams^*

¹From the Departments of Haematology and Lupus Research, St Thomas’ Hospital, London, UK
Kiran Parmar^*

¹From the Departments of Haematology and Lupus Research, St Thomas’ Hospital, London, UK
Graham R. V. Hughes

¹From the Departments of Haematology and Lupus Research, St Thomas’ Hospital, London, UK
Beverley J. Hunt

¹From the Departments of Haematology and Lupus Research, St Thomas’ Hospital, London, UK

Abstract

Summary

The pathogenic mechanism underlying the prothrombotic tendency of Hughes’ or antiphospholipid syndrome (APS) has not been elucidated. Numerous procoagulant mechanisms have been tested including platelet activation, monocyte tissue factor (TF) expression and endothelial cell (EC) activation. There is some evidence for the latter from studies on cultured human umbilical vein endothelial cells (HUVEC). Incubation with antiphospholipid antibodies (aPL) induces EC activation in vitro. We investigated whether there was evidence of EC perturbation in vivo using enzyme-linked immunosorbant assays (ELISAs) for soluble markers of EC dysfunction. Serum and plasma were collected from controls and patients with primary APS and ELISAs performed to quantify soluble vascular cell adhesion molecule (sVCAM), soluble intercellular adhesion molecule-1 (sICAM-1), interleukin-6 (IL-6), endothelin-1 (ET-1), von Willebrand factor (vWF) and soluble tissue factor (sTF). In addition, soluble p-selectin (p-selectin) and vascular endothelial growth factor (VEGF) were measured: the former as a marker of platelet activation, the latter as a potential mediator of TF expression. No significant differences in the levels of blood-borne soluble markers were detected between the patient and control groups except for VEGF and sTF, patients having significantly higher levels of VEGF and sTF than controls (p <0.05). These results suggest plasma soluble tissue factor and VEGF may play a role in the pathogenesis of thrombosis in APS, although the cell of origin of these molecules remains unclear.

Key words

Endothelium - antiphospholipid syndrome

Full Text

References

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