Adipose Tissue Expression of Gelatinases in Mouse Models of Obesity

H. R. Lijnen; E. Maquoi; P. Holvoet; A. Mertens; F. Lupu; P. Morange; M. C. Alessi; I. Juhan-Vague

doi:10.1055/s-0037-1615971

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2001; 85(06): 1111-1116
DOI: 10.1055/s-0037-1615971

Review Article

Schattauer GmbH

Adipose Tissue Expression of Gelatinases in Mouse Models of Obesity

Authors

H. R. Lijnen

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
E. Maquoi

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
P. Holvoet

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
A. Mertens

¹Center for Molecular and Vascular Biology, University of Leuven, Leuven, Belgium
F. Lupu

²Vascular Biology Laboratory, Thrombosis Research Institute, London, UK
P. Morange

³Haematology Laboratory, CHU Timone, Marseille, France
M. C. Alessi

³Haematology Laboratory, CHU Timone, Marseille, France
I. Juhan-Vague

³Haematology Laboratory, CHU Timone, Marseille, France

Abstract

Summary

Following the observation by Brown et al. (Am J Physiol 1997; 272: C937-49) that primary rat adipocytes in culture secrete gelatinase A (MMP-2), we have evaluated gelatinase expression in adipose tissue with the use of mouse models of obesity. Wild-type mice were kept on a standard fat diet (SFD) or on a high fat diet (42% fat, HFD) and genetically obese db/db mice were kept on SFD; gonadal and subcutaneous fat pads were removed and analysed ex vivo. These studies revealed that: 1) the HFD induced adipocyte hypertrophy; 2) after 32 weeks, significantly higher levels of 70 kDa (p <0.05) and 65 kDa proMMP-2 (p < 0.01) were observed in extracts of gonadal fat pads of mice on HFD; 3) the contribution of active MMP-2 to the total level was comparable in SFD and HFD groups (20 to 30%); and 4) gelatinase B (MMP-9) was not consistently detected. These findings were confirmed by gelatin zymography and by mRNA determination using competitive RT-PCR. The presence of MMP-2 in the adipose tissue was confirmed immunologically and its localization in adipocytes revealed by immunogold electron microscopy. The potential functional role of MMP-2 in adipose tissue remains to be determined.

Key words

Obesity - matrix metalloproteinases - gelatinase

Full Text

References

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