Assembly of High Molecular Weight Kininogen and Activation of Prekallikrein on Cell Matrix

G. Motta; Z. Shariat-Madar; F. Mahdi; C. A. M. Sampaio; A. H. Schmaier

doi:10.1055/s-0037-1616141

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2001; 86(03): 840-847
DOI: 10.1055/s-0037-1616141

Review Articles

Schattauer GmbH

Assembly of High Molecular Weight Kininogen and Activation of Prekallikrein on Cell Matrix

Authors

G. Motta

¹Departamento de Bioquímica, Univesidade Federal de São Paulo (UNIFESP-EPM), São Paulo, Brasil, and Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA
Z. Shariat-Madar

¹Departamento de Bioquímica, Univesidade Federal de São Paulo (UNIFESP-EPM), São Paulo, Brasil, and Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA
F. Mahdi

¹Departamento de Bioquímica, Univesidade Federal de São Paulo (UNIFESP-EPM), São Paulo, Brasil, and Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA
C. A. M. Sampaio

¹Departamento de Bioquímica, Univesidade Federal de São Paulo (UNIFESP-EPM), São Paulo, Brasil, and Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA
A. H. Schmaier

¹Departamento de Bioquímica, Univesidade Federal de São Paulo (UNIFESP-EPM), São Paulo, Brasil, and Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA

Abstract

Summary

Investigations determined if extracellular matrix of endothelial cells (EC) is a platform for HK assembly and PK activation. In buffers containing bovine serum albumin, biotin-HK binding to ECV304 cells or their matrix requires ≥ 50 µM added Zn ²⁺. Ortho-phenanthroline or a HK domain 5 peptide blocks HK binding. Binding to umbilical vein EC or matrix, but not ECV304 cells or matrix, is mediated by cytokeratin 1. Biotin-HK binds to ECV304 cells or matrix with a K_d of 15.8 or 9.0 nM and a B_max of 2.6 10⁷ or 2.4 10⁷ sites/cell, respectively. PK activation on ECV304 cells or matrix is blocked by antipain or SBTI and corn trypsin inhibitor partially inhibits kallikrein formation. PK activation occurs on ECV304 cells or matrix prepared without serum or in human factor XII deficient serum, indicating that the PK activator is not factor XIIa. EC matrix promotes plasminogen activation after the assembly of HK, PK and pro-urokinase. These studies indicate that matrix of various EC has the ability to assemble HK allowing for PK activation and subsequent activities.

Abbreviations: EC: endothelial cells, FXII: factor XII, HK: high molecular weight kininogen, HKa: bradykinin-free HK, PK: plasma prekallikrein, Pro-UK: pro-urokinase, uPAR: urokinase plasminogen activator receptor, tcuPA: twochain urokinase, CK1: cytokeratin 1, SBTI: soybean trypsin inhibitor, HUVEC: human umbilical vein endothelial cell, SDS-PAGE: sodium dodecyl sulfatepolyacrylamide gel electrophoresis, CTI: corn trypsin inhibitor, p-APMSF: p-amidinophenylmethylsulfonylfluoride, EBSS: Earle’s Balanced Salt Solution

Keywords

HMW kininogen - prekallikrein - endothelial cells - extracellular matrix - pro-urokinase - plasminogen

Full Text

References

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