Isotopic Determination of Serum Vitamin B12 Level using Sephadex G-25

J. Mantzos; H. Gyftaki; V. Alevizou

doi:10.1055/s-0037-1621339

Nuklearmedizin - NuclearMedicine, Table of Contents

Nuklearmedizin 1967; 06(03): 311-320
DOI: 10.1055/s-0037-1621339

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

Isotopic Determination of Serum Vitamin B₁₂ Level using Sephadex G-25

Détermination isotopique du taux de Vitamine B₁₂ dans le sérum au moyen de Sephadex G-25Isotopenbestimmung des Vitamin-B₁₂-Serumspiegels mittels Sephadex G-25

Authors

J. Mantzos

¹From the Department of Clinical Therapeutics, University of Athens and the Radioisotope Laboratory, Alexandra Hospital, Athens, Greece
H. Gyftaki

¹From the Department of Clinical Therapeutics, University of Athens and the Radioisotope Laboratory, Alexandra Hospital, Athens, Greece
V. Alevizou

¹From the Department of Clinical Therapeutics, University of Athens and the Radioisotope Laboratory, Alexandra Hospital, Athens, Greece

Abstract

Summary

A radioisotope method for the determination of the serum vitamin B₁₂ level is described. The method is based on the principle of saturation analysis and on the ability of Sephadex to separate free from bound vitamin.

We have found the method to be sensitive, reproducible and fairly rapid. Because of its simplicity, it can be used in any laboratory equipped for the use of radioisotopes.

The method was applied to determine the level of Vitamin B₁₂ in 71 normal subjects and in 15 patients with different diseases.

Il est décrit une méthode radioisotopique de la détermination du taux de Vitamine B₁₂ dans le sérum. Cette méthode est basée sur le principe de l’analyse de saturation et de la capacité de Sephadex de séparer les vitamines non-liées et liées.

Nous avons trouvé cette méthode sensible, reproductible et assez rapide. Sa simplicité permet son utilisation dans tout laboratoire installé pour Pemploy de radioisotopes.

La méthode a été utilisée pour la détermination du taux de Vitamine B₁₂ chez 71 sujets normaux et chez 15 sujets affectés de différentes maladies.

Eine Radioisotopenmethode zur Bestimmung des Vitamin-B₁₂-Serumspiegels wird beschrieben. Die Methode beruht auf dem Prinzip der Saturationsanalyse und dem Vermögen von Sephadex, freies von gebundenem Vitamin zu trennen.

Wir fanden die Methode empfindlich, reproduzierbar und ziemlich schnell. Ihre Einfachheit erlaubt ihre Anwendung in jedem Laboratorium, das zur Verwendung von Radioisotopen eingerichtet ist.

Die Methode wurde zur Bestimmung des Vitamin-B₁₂-Spiegels bei 71 normalen Personen und bei 15 Patienten mit verschiedenen Krankheiten verwendet.

Full Text

References

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