Tierarztl Prax Ausg K Kleintiere Heimtiere 2010; 38(04): 211-216
DOI: 10.1055/s-0038-1622855
Original Article
Schattauer GmbH

Solid phase gastric emptying times in the dog measured by 13C-sodium-acetate breath test and 99mTechnetium radioscintigraphy

Messung der Magenentleerung von Festpartikeln beim Hund mittels 13C-Natriumacetat-Atemtest und Szintigraphie
S. Schmitz
1   Veterinary clinic, small animal clinic (internal medicine), Justus-Liebig-University Giessen, Germany
K. Failing
2   Institute for data analysis and biomathematics, Justus-Liebig-University Giessen, Germany
R. Neiger
1   Veterinary clinic, small animal clinic (internal medicine), Justus-Liebig-University Giessen, Germany
› Author Affiliations
Further Information

Publication History

Received:15 February 2010

Accepted after revision:09 April 2010

Publication Date:
05 January 2018 (online)


Objective: The aim of the study was to assess solid gastric emptying via non-invasive 13C-sodium acetate breath test and compare this technique to 99mTechnetium scintigraphy in 12 healthy adult dogs. Material and methods: The dogs were fed a test meal containing either 100 mg 13C-sodium acetate or 150–250 MBq 99mTechnetium albumin colloid. Breath test and scintigraphy were performed on two consecutive days; this set of procedures was repeated in all dogs. Breath samples and scintigrams were obtained at baseline and every 15 minutes for 4 hours, then every 30 minutes for another 2 hours. 25%, 50% and 75% gastric emptying times for breath test (Gt25%b, Gt50%b, Gt75%b) and scintigraphy (Gt25%s, Gt50%s, Gt75%s) were calculated and compared. Results: The mean (± SD) Gt50%b and Gt50%s were 165 (± 28.1) and 71 (± 16.6) minutes, respectively. There was a significant correlation at all three gastric emptying times between breath test and scintigraphy. Conclusion and clinical significance: While gastric emptying times between both methods varied considerably, both methods correlated significantly showing that the gastric emptying breath test can be used to assess gastric emptying times in dogs.


Gegenstand und Ziel dieser Studie war die Bestimmung der Magenentleerungszeit für Futter bei 12 gesunden Hunden mithilfe des nichtinvasiven 13C-Natriumazetat-Atemtests sowie dessen Vergleich mit der 99mTechnetium-Radioszintigraphie. Material und Methoden: Den Hunden wurde eine Testmahlzeit verabreicht, die entweder 100 mg 13C-Natriumazetat oder 150–250 MBq 99mTechnetium (gebunden an Albuminkolloid) enthielt. Atemtests und Szintigraphien wurden an zwei aufeinanderfolgenden Tagen bei allen Hunden durchgeführt. Anschließend erfolgte eine Wiederholung dieser Prozedur bei allen Hunden. Die Entnahme von Atemproben sowie die Anfertigung von Szintigrammen fanden zum Zeitpunkt 0 (direkt nach der Futteraufnahme) sowie alle 15 Minuten nach der Futteraufnahme für 4 Stunden und dann alle 30 Minuten für weitere 2 Stunden statt. Die Zeit der Entleerung von jeweils 25%, 50% and 75% des Mageninhaltes wurde sowohl für den Atemtest (Gt25%b, Gt50%b, Gt75%b) als auch die Szintigraphie (Gt25%s, Gt50%s, Gt75%s) bestimmt und verglichen. Ergebnisse: Die mittleren Magenentleerungszeiten (Mittelwert ± Standardabweichung) Gt50%b und Gt50%s lagen bei 165 ± 28,1 bzw. 71 ± 16,6 Minuten. Zwischen dem Atemtest und der Szintigraphie bestand zu allen drei Zeitpunkten der Magenentleerung eine signifikante Korrelation. Schlussfolgerung und klinische Relevanz: Wenngleich die Magenentleerungszeiten zwischen den beiden Methoden variierten, korrelierten sie signifikant, sodass der Atemtest zur Bestimmung der Magenentleerungzeiten beim Hund eingesetzt werden kann.

  • References

  • 1 Ando H, Mochiki E, Ohno T, Kogure N, Tanaka N, Tabe Y. et al. Effect of distal subtotal gastrectomy with preservation of the celiac branch of the vagus nerve to gastrointestinal function: an experimental study in conscious dogs. Ann Surg 2008; 247 (06) 976-986.
  • 2 Armbrust LJ, Hoskinson JJ, Lora-Michiels M, Milliken GA. Gastric emptying in cats using foods varying in fiber content and kibble shapes. Vet Radiol Ultrasound 2003; 44 (03) 339-343.
  • 3 Bluck LJ, Coward WA. Measurement of gastric emptying by the 13C-octa -noate breath test – rationalization with scintigraphy. Physiol Meas 2006; 27 (03) 279-289.
  • 4 Bourreau J, Hernot D, Bailhache E, Weber M, Ferchaud V, Biourge V. et al. Gastric emptying rate is inversely related to body weight in dog breeds of different sizes. J Nutr 2004; 134 (Suppl. 08) 2039S-2041S.
  • 5 Braden B, Caspary W, Borner N, Vinson B, Schneider AR. Clinical effects of STW 5 (Iberogast) are not based on acceleration of gastric emptying in patients with functional dyspepsia and gastroparesis. Neurogastroenterol Motil 2009; 21 (06) 632-638. e25
  • 6 Chaudhuri TK. Use of 99mTc-DTPA for measuring gastric emptying time. J Nucl Med 1974; 15 (06) 391-395.
  • 7 Cullen JJ, Titler S, Ephgrave KS, Hinkhouse MM. Gastric emptying of liquids and postprandial pancreatobiliary secretion are temporarily impaired during endotoxemia. Dig Dis Sci 1999; 44 (11) 2172-2177.
  • 8 Donohoe KJ, Maurer AH, Ziessman HA, Urbain JL, Royal HD. Procedure guideline for gastric emptying and motility. Society of Nuclear Medicine. J Nucl Med 1999; 40 (07) 1236-1239.
  • 9 Dormehl IC, du Plessis M, Maree M, Pilloy WJ. A comparison of 111In-labelled polymer beads and 99mTc-Sn-colloid as solid food and semi-solid food tracers for scintigraphic gastric emptying studies. Nuklearmedizin 1986; 25 (02) 50-54.
  • 10 Fioramonti J, Bueno L. Gastrointestinal myoelectric activity disturbances in gastric ulcer disease in rats and dogs. Dig Dis Sci 1980; 25 (08) 575-580.
  • 11 Ghoos YF, Maes BD, Geypens BJ, Mys G, Hiele MI, Rutgeerts PJ. et al. Measurement of gastric emptying rate of solids by means of a carbon-labeled octanoic acid breath test. Gastroenterology 1993; 104 (06) 1640-1647.
  • 12 Glickman LT, Glickman NW, Schellenberg DB, Raghavan M, Lee T. Non-dietary risk factors for gastric dilatation-volvulus in large and giant breed dogs. J Am Vet Med Assoc 2000; 217 (10) 1492-1499.
  • 13 Goetze O, Fox M, Kwiatek MA, Treier R, Schwizer W, Thumshirn M. et al. Effects of postgastric 13C-acetate processing on measurement of gastric emptying: a systematic investigation in health. Neurogastroenterol Motil 2009; 21 (10) 1047-e85.
  • 14 Goggin JM, Hoskinson JJ, Butine MD, Foster LA, Myers NC. Scintigraphic assessment of gastric emptying of canned and dry diets in healthy cats. Am J Vet Res 1998; 59 (04) 388-392.
  • 15 Gondim FA, de Oliveira GR, Thomas FP. Upper gastrointestinal motility changes following spinal cord injury. Neurogastroenterol Motil 2010; 22 (01) 2-6.
  • 16 Griffith GH, Owen GM, Kirkman S, Shields R. Measurement of rate of gastric emptying using chromium-51. Lancet 1966; 1 7449 1244-1245.
  • 17 Hellmig S, Von Schoning F, Gadow C, Katsoulis S, Hedderich J, Folsch UR. et al. Gastric emptying time of fluids and solids in healthy subjects determined by 13C breath tests: influence of age, sex and body mass index. J Gastroenterol Hepatol 2006; 21 (12) 1832-1838.
  • 18 Hoskinson JJ, Goggin JM, Butine MD. Evaluation of solid-phase labels for gastric emptying studies in cats. J Nucl Med 1997; 38 (03) 495-499.
  • 19 Hotokezaka M, Mentis EP, Patel SP, Combs MJ, Teates CD, Schirmer BD. Recovery of gastrointestinal tract motility and myoelectric activity change after abdominal surgery. Arch Surg 1997; 132 (04) 410-417.
  • 20 Iwanaga Y, Wen J, Thollander MS, Kost LJ, Thomforde GM, Allen RG. et al. Scintigraphic measurement of regional gastrointestinal transit in the dog. Am J Physiol 1998; 275 5 Pt 1 G904-9010.
  • 21 Johnson CS, Fales-Williams AJ, Reimer SB, Lotsikas PJ, Haynes JS. Fibrosing gastrointestinal leiomyositis as a cause of chronic intestinal pseudo-obstruction in an 8-month-old dog. Vet Pathol 2007; 44 (01) 106-109.
  • 22 Keller J, Andresen V, Wolter J, Layer P, Camilleri M. Influence of clinical parameters on the results of 13C-octanoic acid breath tests: examination of different mathematical models in a large patient cohort. Neurogastroenterol Motil 2009; 21 (10) 1039-e83.
  • 23 Maes BD, Mys G, Geypens BJ, Evenepoel P, Ghoos YF, Rutgeerts PJ. Gastric emptying flow curves separated from carbon-labeled octanoic acid breath test results. Am J Physiol 1998; 275 1 Pt 1 169-75.
  • 24 Mion F, Ecochard R, Guitton J, Ponchon T. (13)CO (2) breath tests: comparison of isotope ratio mass spectrometry and non-dispersive infrared spectrometry results. Gastroenterol Clin Biol 2001; 25 (04) 375-379.
  • 25 Parkman HP, Harris AD, Krevsky B, Urbain JL, Maurer AH, Fisher RS. Gastroduodenal motility and dysmotility: an update on techniques available for evaluation. Am J Gastroenterol 1995; 90 (06) 869-892.
  • 26 Pouteau EB, Mariot SM, Martin LJ, Dumon HJ, Mabon FJ, Krempf MA. et al. Rate of carbon dioxide production and energy expenditure in fed and food-deprived adult dogs determined by indirect calorimetry and isotopic methods. Am J Vet Res 2002; 63 (01) 111-118.
  • 27 Punkkinen J, Konkka I, Punkkinen O, Korppi-Tommola T, Farkkila M, Koskenpato J. Measuring gastric emptying: comparison of 13C-octanoic acid breath test and scintigraphy. Dig Dis Sci 2006; 51 (02) 262-267.
  • 28 Sagawa K, Li F, Liese R, Sutton SC. Fed and fasted gastric pH and gastric residence time in conscious beagle dogs. J Pharm Sci 2009; 98 (07) 2494-2500.
  • 29 Sanaka M, Yamamoto T, Ishii T, Kuyama Y. The Wagner-Nelson method can generate an accurate gastric emptying flow curve from CO2 data obtained by a 13C-labeled substrate breath test. Digestion 2004; 69 (02) 71-78.
  • 30 Schwabe AD, Bennett LR, Bowman LP. Octanoic Acid Absorption and Oxi -dation in Humans. J Appl Physiol 1964; 19: 335-337.
  • 31 Sutton DG, Bahr A, Preston T, Christley RM, Love S, Roussel AJ. Validation of the 13C-octanoic acid breath test for measurement of equine gastric emptying rate of solids using radioscintigraphy. Equine Vet J 2003; 35 (01) 27-33.
  • 32 Szarka LA, Camilleri M, Vella A, Burton D, Baxter K, Simonson J. et al. A stable isotope breath test with a standard meal for abnormal gastric emptying of solids in the clinic and in research. Clin Gastroenterol Hepatol 2008; 6 (06) 635-643. e1
  • 33 Takeda M, Mizutani Y, Yamano M, Tsukamoto K, Suzuki T. Gastric emptying in diabetic gastroparetic dogs: effects of SK-951, a novel prokinetic agent. Pharmacology 2001; 62 (01) 23-28.
  • 34 Vandenbrom WE, Happe RP. Gastric-emptying of a radionuclide-labeled test meal in healthy dogs – a new mathematical analysis and reference va -lues. Am J Vet Res 1986; 47 (10) 2170-2174.
  • 35 Verbeke K. Will the 13C-octanoic acid breath test ever replace scintigraphy as the gold standard to assess gastric emptying?. Neurogastroenterol Motil 2009; 21 (10) 1013-1016.
  • 36 Walter MC, Goldschmidt MH, Stone EA, Dougherty JF, Matthiesen DT. Chronic hypertrophic pyloric gastropathy as a cause of pyloric obstruction in the dog. J Am Vet Med Assoc 1985; 186 (02) 157-161.
  • 37 Washabau RJ. Gastrointestinal motility disorders and gastrointestinal prokinetic therapy. Vet Clin North Am Small Anim Pract 2003; 33 (05) 1007-1028.
  • 38 Wyse CA, McLellan J, Dickie AM, Sutton DG, Preston T, Yam PS. A review of methods for assessment of the rate of gastric emptying in the dog and cat: 1898–2002. J Vet Intern Med 2003; 17 (05) 609-621.
  • 39 Wyse CA, Preston T, Love S, Morrison DJ, Cooper JM, Yam PS. Use of the 13C-octanoic acid breath test for assessment of solid-phase gastric emptying in dogs. Am J Vet Res 2001; 62 (12) 1939-1944.
  • 40 Zahn A, Langhans CD, Hoffner S, Haberkorn U, Rating D, Haass M. et al. Measurement of gastric emptying by 13C-octanoic acid breath test versus scintigraphy in diabetics. Z Gastroenterol 2003; 41 (05) 383-390.