Methods Inf Med 2000; 39(02): 175-178
DOI: 10.1055/s-0038-1634269
Original Article
Schattauer GmbH

Quantitative Blood Velocity Mapping in Glomerular Capillaries by in vivo Observation with an Intravital Videomicroscope

Y. Ogasawara
1  Dept. of Medical Engineering, Kawasaki Medical School, Kurashiki, Japan
,
K. Takehara
2  Dept. of Electronic Engineering, Okayama University of Science, Okayama, Japan
,
T. Yamamoto
1  Dept. of Medical Engineering, Kawasaki Medical School, Kurashiki, Japan
,
R. Hashimoto
2  Dept. of Electronic Engineering, Okayama University of Science, Okayama, Japan
,
H. Nakamoto
1  Dept. of Medical Engineering, Kawasaki Medical School, Kurashiki, Japan
,
F. Kajiya
1  Dept. of Medical Engineering, Kawasaki Medical School, Kurashiki, Japan
› Author Affiliations
Further Information

Publication History

Publication Date:
07 February 2018 (online)

Abstract:

We have evaluated rat glomerular microcirculation under in-vivo condition with an intravital videomicroscope. To measure erythrocyte velocities, a line segment was set along the glomerular capillaries in time-sequential videotaped images and then a spatiotemporal image was constructed along that segment. The angle of striped pattern in the spatio-temporal image which reflects the erythrocyte velocity, was estimated to compute erythrocyte velocity vector mapping. We found that erythrocyte velocities in rat glomeruli were significantly faster in diabetic rats than in control rats (p <0.05). In conclusion, we have succeeded in quantitatively evaluating the in-vivo renal microcirculation with our videomicroscope system and by spatiotemporal image analyzing method.